Scattered Light Imaging Enables Real-Time Monitoring of Label-Free Nanoparticles and Fluorescent Biomolecules in Live Cells
文献类型：期刊论文
| 作者 | Wang, Fengbang3,6; Chen, Bolei4; Yan, Bing5; Yin, Yongguang3; Hu, Ligang; Liang, Yong1,4; Song, Maoyong3,6 ; Jiang, Guibin6
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| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
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| 出版日期 | 2019-09-11 |
| 卷号 | 141期号:36页码:14043-14047 |
| ISSN号 | 0002-7863 |
| 英文摘要 | Simultaneously monitoring label-free nanoparticles (NPs) and fluorescent biomolecules inside the live cell in real time is challenging because both imaging methods require different instrumentation and measuring principles. Here we report a novel scattered light imaging (SLi) technique that allows label-free NPs to be monitored using a conventional confocal microscope. The method shows a high spatial resolution and can distinguish label-free silver nanoparticles (AgNPs) with a 10 nm size difference in live cells. We performed SLi to observe the uptake, movement, distribution, and transformation of AgNPs in live cells at a single-particle level. The method is applicable to accurately track the localization of a variety of nanomaterials inside the cell. With this approach, label-free NP and fluorescent-labeled biomolecules are imaged simultaneously making it possible to real-time monitor nanobio interactions. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42585]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Guangzhou Univ, Minist Educ, Key Lab Water Qual & Conservat Pearl River Delta, Inst Environm Res Greater Bay, Guangzhou 510006, Guangdong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 4.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Environm Nanotechnol & Hlth Effects, Beijing 100085, Peoples R China 5.Jianghan Univ, Hubei Key Lab Environm & Hlth Effects Persistent, Wuhan 430056, Hubei, Peoples R China 6.Jianghan Univ, Inst Environm & Hlth, Wuhan 430056, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Fengbang,Chen, Bolei,Yan, Bing,et al. Scattered Light Imaging Enables Real-Time Monitoring of Label-Free Nanoparticles and Fluorescent Biomolecules in Live Cells[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2019,141(36):14043-14047. |
| APA | Wang, Fengbang.,Chen, Bolei.,Yan, Bing.,Yin, Yongguang.,Hu, Ligang.,...&Jiang, Guibin.(2019).Scattered Light Imaging Enables Real-Time Monitoring of Label-Free Nanoparticles and Fluorescent Biomolecules in Live Cells.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,141(36),14043-14047. |
| MLA | Wang, Fengbang,et al."Scattered Light Imaging Enables Real-Time Monitoring of Label-Free Nanoparticles and Fluorescent Biomolecules in Live Cells".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 141.36(2019):14043-14047. |
入库方式： OAI收割
来源：生态环境研究中心
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