Targeted live-cell nuclear delivery of the DNA 'light-switching' Ru(II) complex via ion-pairing with chlorophenolate counter-anions: the critical role of binding stability and lipophilicity of the ion-pairing complexes
文献类型：期刊论文
| 作者 | Chao, Xi-Juan; Tang, Miao; Huang, Rong; Huang, Chun-Hua; Shao, Jie; Yan, Zhu-Ying; Zhu, Ben-Zhan |
| 刊名 | NUCLEIC ACIDS RESEARCH
 |
| 出版日期 | 2019-11-18 |
| 卷号 | 47期号:20页码:10520-10528 |
| ISSN号 | 0305-1048 |
| 英文摘要 | We have found recently that nuclear uptake of the cell-impermeable DNA light-switching Ru(II)-polypyridyl cationic complexes such as [Ru(bpy)(2)(dppz)]Cl-2 was remarkably enhanced by pentachlorophenol (PCP), by forming ion-pairing complexes via a passive diffusion mechanism. However, it is not clear whether the enhanced nuclear uptake of [Ru(bpy)(2)(dppz)](2+) is only limited to PCP, or it is a general phenomenon for other highly chlorinated phenols (HCPs); and if so, what are the major physicochemical factors in determining nuclear uptake? Here, we found that the nuclear uptake of [Ru(bpy)(2)(dppz)](2+) can also be facilitated by other two groups of HCPs including three tetrachlorophenol (TeCP) and six trichlorophenol (TCP) isomers. Interestingly and unexpectedly, 2,3,4,5-TeCP was found to be the most effective one for nuclear delivery of [Ru(bpy)(2)(dppz)](2+), which is even better than the most-highly chlorinated PCP, and much better than its two other TeCP isomers. Further studies showed that the nuclear uptake of [Ru(bpy)(2)(dppz)](2+) was positively correlated with the binding stability, but to our surprise, inversely correlated with the lipophilicity of the ion-pairing complexes formed between [Ru(bpy)(2)(dppz)]Cl-2 and HCPs. These findings should provide new perspectives for future investigations on using ion-pairing as an effective method for delivering other bio-active metal complexes into their intended cellular targets. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42602]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Univ Chinese Acad Sci, Beijing 100085, Peoples R China 2.[Chao, Xi-Juan 3.Tang, Miao 4.Huang, Rong 5.Huang, Chun-Hua 6.Shao, Jie 7.Yan, Zhu-Ying 8.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 9.Sun Yat Sen Univ, Sch Chem, MOE Key Lab Bioinorgan & Synthet Chem, Guangzhou 510275, Guangdong, Peoples R China 10.Oregon State Univ, Linus Pauling Inst, Corvallis, OR 97331 USA |
| 推荐引用方式 GB/T 7714 | Chao, Xi-Juan,Tang, Miao,Huang, Rong,et al. Targeted live-cell nuclear delivery of the DNA 'light-switching' Ru(II) complex via ion-pairing with chlorophenolate counter-anions: the critical role of binding stability and lipophilicity of the ion-pairing complexes[J]. NUCLEIC ACIDS RESEARCH,2019,47(20):10520-10528. |
| APA | Chao, Xi-Juan.,Tang, Miao.,Huang, Rong.,Huang, Chun-Hua.,Shao, Jie.,...&Zhu, Ben-Zhan.(2019).Targeted live-cell nuclear delivery of the DNA 'light-switching' Ru(II) complex via ion-pairing with chlorophenolate counter-anions: the critical role of binding stability and lipophilicity of the ion-pairing complexes.NUCLEIC ACIDS RESEARCH,47(20),10520-10528. |
| MLA | Chao, Xi-Juan,et al."Targeted live-cell nuclear delivery of the DNA 'light-switching' Ru(II) complex via ion-pairing with chlorophenolate counter-anions: the critical role of binding stability and lipophilicity of the ion-pairing complexes".NUCLEIC ACIDS RESEARCH 47.20(2019):10520-10528. |
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来源：生态环境研究中心
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