2,3,7,8-Tetrachlorodibenzo-p-dioxin promotes migration ability of primary cultured rat astrocytes via aryl hydrocarbon receptor
文献类型：期刊论文
| 作者 | Chen, Yangsheng; Sha, Rui; Xu, Li; Xia, Yingjie; Liu, Yiyun; Li, Xuejun; Xie, Heidi Qunhui; Tang, Naijun; Zhao, Bin |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES
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| 出版日期 | 2019-02 |
| 卷号 | 76页码:368-376 |
| 关键词 | 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) Astrocyte Migration Gene expression Aryl hydrocarbon receptor (AhR) pathway |
| ISSN号 | 1001-0742 |
| 英文摘要 | Emerging evidence showed that 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) could induce expression of certain reactivation-associated genes in astrocytes, however, the consequent cellular effects and molecular mechanisms are still unclear. During the process of astrocyte reactivation, migration is a critical cellular event In the present study, we employed wound-healing assay and Transwell (R) motility assay to explore the effects of TCDD on cell migration in primary cultured rat cortical astrocytes. We found that upon TCDD treatments at relative low concentrations (10(-)(10) and/or 10(-9) mol/L), the ability of primary astrocytes to migrate horizontally and vertically was promoted. In line with this cellular effect, the mRNA expression of two pro- migratory genes, including cell division cycle 42 (CDC42) and matrix metalloproteinase 2 (MMP2) was induced by TCDD treatment Dioxin exerts its toxic effects mainly through aryl hydrocarbon receptor (AhR) pathway. So the role of AhR pathway in the pro-migratory effects of TCDD was examined using an AhR antagonist, CH223191. We found that application of CH223191 significantly reversed the pro-migratory effects of TCDD. Interestingly, the basal ability of horizontal migration as well as basal levels of CDC42 and MMP2 expression were dramatically reduced suggesting a possible physiological role of AhR in maintaining the endogenous migration ability of the primary astrocytes. These findings support the notion that dioxin promotes astrocyte reactivation at molecular and cellular levels. (C) 2018 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42881]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Tianjin Med Univ, Dept Occupat & Environm Hlth, Tianjin 300070, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Yangsheng,Sha, Rui,Xu, Li,et al. 2,3,7,8-Tetrachlorodibenzo-p-dioxin promotes migration ability of primary cultured rat astrocytes via aryl hydrocarbon receptor[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2019,76:368-376. |
| APA | Chen, Yangsheng.,Sha, Rui.,Xu, Li.,Xia, Yingjie.,Liu, Yiyun.,...&Zhao, Bin.(2019).2,3,7,8-Tetrachlorodibenzo-p-dioxin promotes migration ability of primary cultured rat astrocytes via aryl hydrocarbon receptor.JOURNAL OF ENVIRONMENTAL SCIENCES,76,368-376. |
| MLA | Chen, Yangsheng,et al."2,3,7,8-Tetrachlorodibenzo-p-dioxin promotes migration ability of primary cultured rat astrocytes via aryl hydrocarbon receptor".JOURNAL OF ENVIRONMENTAL SCIENCES 76(2019):368-376. |
入库方式： OAI收割
来源：生态环境研究中心
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