3D printed gel electrophoresis device coupling with ICP-MS for online separation and detection of metalloproteins
文献类型：期刊论文
| 作者 | Wang, Dingyi; He, Bin; Yan, Xueting; Nong, Qiying; Wang, Chao ; Jiang, Jie; Hu, Ligang; Jiang, Guibin
|
| 刊名 | TALANTA
 |
| 出版日期 | 2019-05-15 |
| 卷号 | 197页码:145-150 |
| 关键词 | 3D printing Gel electrophoresis Metalloprotein ICP-MS |
| ISSN号 | 0039-9140 |
| 英文摘要 | We successfully developed a strategy to combine a customized gel electrophoresis device with ICP-MS for online separation and detection of metalloproteins. The self-designed horizontal column gel electrophoresis device was rapidly and easily fabricated in the laboratory via 3D printing with a low cost. The feasibility of 3D printing to fabricate this device was investigated by offline separation of commercial protein standards. And a better separation efficiency was found when using gel tubes printed with higher printing precision. As a proof-of-concept, the performance of the whole system is demonstrated by online separation and detection of both iodinated protein standards and proteins in rat blood plasma samples. Benefits from 3D printing, customized modification or further optimization can be readily achieved for a better protein separation and detection efficiency. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42882]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China 2.Shenzhen Ctr Dis Control & Prevent, Shenzhen 518055, Peoples R China 3.Jianghan Univ, Inst Environm & Hlth, Wuhan 430056, Hubei, Peoples R China 4.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Dingyi,He, Bin,Yan, Xueting,et al. 3D printed gel electrophoresis device coupling with ICP-MS for online separation and detection of metalloproteins[J]. TALANTA,2019,197:145-150. |
| APA | Wang, Dingyi.,He, Bin.,Yan, Xueting.,Nong, Qiying.,Wang, Chao.,...&Jiang, Guibin.(2019).3D printed gel electrophoresis device coupling with ICP-MS for online separation and detection of metalloproteins.TALANTA,197,145-150. |
| MLA | Wang, Dingyi,et al."3D printed gel electrophoresis device coupling with ICP-MS for online separation and detection of metalloproteins".TALANTA 197(2019):145-150. |
入库方式： OAI收割
来源：生态环境研究中心
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