A pH-insensitive near-infrared fluorescent probe for wash-free lysosome-specific tracking with long time during physiological and pathological processes
文献类型：期刊论文
| 作者 | Chao, Xi-Juan; Pan, Zheng-Yin; Sun, Li-Li; Tang, Miao; Wang, Kang-Nan; Mao, Zong-Wan |
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2019-04-15 |
| 卷号 | 285页码:156-163 |
| 关键词 | Near-infrared Off-on Wash-free Lysosome-specific imaging pH-insensitivity Long-term tracking |
| 英文摘要 | A novel dicyanomethylene-4H-pyran based lysosome-specific near-infrared (NIR) probe, DCM-ML, is reported. It exhibits very weak fluorescence (Phi = 0.007) in aqueous solution due to the energy relaxation from intramolecular rotation; but a large off-on NIR emission (20-folds) after specifically labeling lysosomes due to the local high viscosity instead of the local pH by both one-and two-photon excitation microscopy. Therefore, the imaging ability of DCM-ML will not confront the problems suffered by the currently used and reported pH-dependent lysosome probes (e.g., once lysosomal pH increases, their fluorescence gets quenched); The pH-independent feature of DCM-ML is further demonstrated to be a great advantage for tracing lysosomes'movements in a relatively long time, even during cellular processes when lysosomes are suffered from external stimulations that induce pH increase and apoptosis. In addition to the characteristics of pH-insensitivity and off-on NIR emission, DCM-ML also possesses characteristics of excellent membrane permeability, high selectivity, low cytotoxicity, good photostability and large Stokes shift (> 200 nm), which qualify it as a superior wash-free NIR probe for tracking dynamic changes of lysosomes under both physiological and toxicological conditions. Hence, we believe DCM-ML may find a great potential of application in the future. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42886]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Chinese Acad Sci, Beijing 100085, Peoples R China 2.Chinese Acad Sci, State Key Lab Environm Chem & Ecotoxicol, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China 3.Sun Yat Sen Univ, Sch Chem, MOE Key Lab Bioinorgan & Synthet Chem, Guangzhou 510275, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chao, Xi-Juan,Pan, Zheng-Yin,Sun, Li-Li,et al. A pH-insensitive near-infrared fluorescent probe for wash-free lysosome-specific tracking with long time during physiological and pathological processes[J]. SENSORS AND ACTUATORS B-CHEMICAL,2019,285:156-163. |
| APA | Chao, Xi-Juan,Pan, Zheng-Yin,Sun, Li-Li,Tang, Miao,Wang, Kang-Nan,&Mao, Zong-Wan.(2019).A pH-insensitive near-infrared fluorescent probe for wash-free lysosome-specific tracking with long time during physiological and pathological processes.SENSORS AND ACTUATORS B-CHEMICAL,285,156-163. |
| MLA | Chao, Xi-Juan,et al."A pH-insensitive near-infrared fluorescent probe for wash-free lysosome-specific tracking with long time during physiological and pathological processes".SENSORS AND ACTUATORS B-CHEMICAL 285(2019):156-163. |
入库方式： OAI收割
来源：生态环境研究中心
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