A simple aptamer molecular beacon assay for rapid detection of aflatoxin B1
文献类型：期刊论文
| 作者 | Wang, Chao ; Sun, Linlin; Zhao, Qiang
|
| 刊名 | CHINESE CHEMICAL LETTERS
 |
| 出版日期 | 2019-05 |
| 卷号 | 30期号:5页码:1017-1020 |
| 关键词 | Aptamer Aflatoxin B1 Fluorescence Molecular beacons Mycotoxin Sensor |
| ISSN号 | 1001-8417 |
| 英文摘要 | Aflatoxin B1 (AFB1) is a highly toxic mycotoxin, and rapid and sensitive detection of AFB1 is in demand for food safety and environmental analysis. Here we described a simple aptamer molecular beacon assay for rapid detection of aflatoxin B1 (AFB1) by using an aptamer with a fluorescein (FAM) label at the 5' end and a fluorescence quencher (black hole quencher 1, BHQ1) at the 3' end. In the presence of AFB1, the aptamer probe bound with AFB1 and induced a hairpin structure, drawing FAM and BHQ1 into close proximity and leading to fluorescence quenching. This assay allowed for a detection limit of 3.9 nmol/L and a dynamic range from 3.9 nmol/L, to 4 mu mol/L. Specificity test showed other mycotoxins including ochratoxin A, ochratoxin B, fumonisin B1, fumonisin B2, and zearalenone had negligible influence on detection of AFB1. AFB1 spiked in diluted liquor wine, methanol, or corn flour samples was successfully detected by using this aptamer probe, and the assay showed potential for real sample analysis. (C) 2019 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42887]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Chao,Sun, Linlin,Zhao, Qiang. A simple aptamer molecular beacon assay for rapid detection of aflatoxin B1[J]. CHINESE CHEMICAL LETTERS,2019,30(5):1017-1020. |
| APA | Wang, Chao,Sun, Linlin,&Zhao, Qiang.(2019).A simple aptamer molecular beacon assay for rapid detection of aflatoxin B1.CHINESE CHEMICAL LETTERS,30(5),1017-1020. |
| MLA | Wang, Chao,et al."A simple aptamer molecular beacon assay for rapid detection of aflatoxin B1".CHINESE CHEMICAL LETTERS 30.5(2019):1017-1020. |
入库方式： OAI收割
来源：生态环境研究中心
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