A unique Pb-binding flagellin as an effective remediation tool for Pb contamination in aquatic environment
文献类型：期刊论文
| 作者 | Chen, Baowei; Fang, Linchuan; Yan, Xueting; Zhang, Aiqian; Chen, Ping; Luan, Tiangang; Hu, Ligang; Jiang, Guibin
|
| 刊名 | JOURNAL OF HAZARDOUS MATERIALS
 |
| 出版日期 | 2019-02-05 |
| 卷号 | 363页码:34-40 |
| 关键词 | Lead Biosorption Metal-binding protein Microbes |
| ISSN号 | 0304-3894 |
| 英文摘要 | Metal contaminants present persistent and deleterious threats to environmental ecosystems and human health. Microorganisms can rapidly develop protective mechanisms against metal toxicity, such as metallothionein production. The identification of biological factors related to these protective mechanisms is essential for effective metal remediation. This study presents a robust pathway to rapidly locate and characterize a Pb-binding flagellin in Serratia Se1998, which can bind Pb at a 16:1 Pb: protein ratio. A column gel electrophoresis system hyphenated with inductively coupled plasma mass spectrometry (ICP MS) was constructed to efficiently separate and identify Pb-binding proteins from the whole bacterial proteome. PCR and transgenic assays were used to elucidate the exact sequences and biological function of Pb-binding proteins and heterogeneous expression of Pb-binding flagellin in E. colt could significantly enhance Pb removal from aqueous solution by approximately 45%. This method provides a benchmark procedure to rapidly identify biological factors responsible for metal bio-sorption. Identification of this unique Pb-binding flagellin highlights that microorganisms can survive high metal stresses due to various complex biological pathways for metal detoxification and remediation. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42888]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Northwest A&F Univ, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Shaanxi, Peoples R China 2.Sun Yat Sen Univ, Sch Marine Sci, Guangdong Prov Key Lab Marine Resources & Coastal, Guangzhou 510275, Guangdong, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, POB 2871, Beijing 100085, Peoples R China 4.Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China 5.Sun Yat Sen Univ, Sch Environm Sci & Engn, Guangzhou 510275, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Baowei,Fang, Linchuan,Yan, Xueting,et al. A unique Pb-binding flagellin as an effective remediation tool for Pb contamination in aquatic environment[J]. JOURNAL OF HAZARDOUS MATERIALS,2019,363:34-40. |
| APA | Chen, Baowei.,Fang, Linchuan.,Yan, Xueting.,Zhang, Aiqian.,Chen, Ping.,...&Jiang, Guibin.(2019).A unique Pb-binding flagellin as an effective remediation tool for Pb contamination in aquatic environment.JOURNAL OF HAZARDOUS MATERIALS,363,34-40. |
| MLA | Chen, Baowei,et al."A unique Pb-binding flagellin as an effective remediation tool for Pb contamination in aquatic environment".JOURNAL OF HAZARDOUS MATERIALS 363(2019):34-40. |
入库方式： OAI收割
来源：生态环境研究中心
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