Cytotoxicity and autophagy induction by graphene quantum dots with different functional groups
文献类型：期刊论文
| 作者 | Xie, Yichun; Wan, Bin; Yang, Yu; Cui, Xuejing; Xin, Yan; Guo, Liang-Hong |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES
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| 出版日期 | 2019-03 |
| 卷号 | 77页码:198-209 |
| ISSN号 | 1001-0742 |
| 关键词 | Graphene quantum dots Cytotoxicity Autophagy Surface functional group |
| 英文摘要 | Craphene quantum dots (CQDs) possess great potential in various applications due to their superior physicochemical properties and wide array of available surface modifications. However, the toxicity of GQDs has not been systematically assessed, thus hindered their further development; especially, the risk of surface modifications of GQDs is largely unknown. In this study, we employed a lung carcinoma A549 cells as the model to investigate the cytotoxicity and autophagy induction of three types GQDs, including cGQDs COOH-CQDs), hGQDs (OH-GQDs), and aGQDs (NH2-GQDs). The results showed hGQDs was the most toxic, as significant cell death was induced at the concentration of 100 mu g/mL, determining by WST-1 assay as well as Annexin-V-FITC/PI apoptosis analysis, whereas cGQDs and aGQDs were non-cytotoxic within the measured concentration. Autophagy detection was performed by TEM examination, LC3 fluorescence tracking, and Westernblot. Both aGQDs and hGQDs induced cellular autophagy to various degrees except for cGQDs. Further analysis on autophagy pathways indicated all GQDs significantly activated p-p38MAPK; p-ERK1/2 was inhibited by aGQDs and hGQDs but activated by cGQDs. p-JNK was inhibited by aGQDs and cGQDs, while activated by hGQDs. Simultaneously, Akt was activated by hGQDs but inhibited by aGQDs. Inhibition of autophagy by 3-MA significantly increased the cytotoxicity of CQDs, suggesting that autophagy played a protective role against the toxicity of GQDs. In conclusion, cGQDs showed excellent biocompatibility and may be considered for biological applications. Autophagy induction may be included in the health risk assessment of GQDs as it reflects the stress status which may eventually lead to diseases. (C) 2018 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42922]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Natl Ctr Nanosci & Technol China, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Jianghan Univ, Inst Environm & Hlth, Wuhan 430056, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie, Yichun,Wan, Bin,Yang, Yu,et al. Cytotoxicity and autophagy induction by graphene quantum dots with different functional groups[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2019,77:198-209. |
| APA | Xie, Yichun,Wan, Bin,Yang, Yu,Cui, Xuejing,Xin, Yan,&Guo, Liang-Hong.(2019).Cytotoxicity and autophagy induction by graphene quantum dots with different functional groups.JOURNAL OF ENVIRONMENTAL SCIENCES,77,198-209. |
| MLA | Xie, Yichun,et al."Cytotoxicity and autophagy induction by graphene quantum dots with different functional groups".JOURNAL OF ENVIRONMENTAL SCIENCES 77(2019):198-209. |
入库方式： OAI收割
来源：生态环境研究中心
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