Discovery of CRR1-targeted copper deficiency response in Chlamydomonas reinhardtii exposed to silver nanoparticles
文献类型：期刊论文
| 作者 | Wang, Songshan; Lv, Jitao; Zhang, Shuzhen |
| 刊名 | NANOTOXICOLOGY
 |
| 出版日期 | 2019-04-21 |
| 卷号 | 13期号:4页码:447-454 |
| 关键词 | Silver nanoparticles (AgNPs) copper response regulator 1 copper deficiency responsive genes Chlamydomonas reinhardtii |
| ISSN号 | 1743-5390 |
| 英文摘要 | The molecular mechanisms behind the adaptive responses for interactions between organisms and nanoparticles, such as silver nanoparticles (AgNPs), are of great concern. In this study, the transcriptome of freshwater alga Chlamydomonas reinhardtii was characterized via RNA sequencing (RNA-seq) after exposure to a nontoxic concentration of AgNPs (0.5mg/L). The cytochrome c6 (CYC6) and ferredoxin-5 (FDX5) genes were identified with the greatest increase in expression level, which were indications of the copper deficiency in the algae. Gene set enrichment analysis also showed significant enrichment of copper deficiency responsive genes in the transcriptome of algae exposed to AgNPs. These results indicated that AgNPs induced a copper deficiency response in algae, and the excessive intracellular copper content suggested this was due to functional copper deficiency. This deficiency response was further validated to be regulated by transcription factor CRR1 (copper response regulator 1) according to the assays on the mutant strain with defect of CRR1. To the best of our knowledge, this is the first corroboration of a CRR1-targeted copper deficiency response in algae following AgNP exposure. Given the function of copper in fundamental metabolic pathways, such as photosynthesis and respiration, we propose a potential role of CRR1-targeted copper deficiency as an adaptation of algae after exposure to AgNPs. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42929]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Songshan,Lv, Jitao,Zhang, Shuzhen. Discovery of CRR1-targeted copper deficiency response in Chlamydomonas reinhardtii exposed to silver nanoparticles[J]. NANOTOXICOLOGY,2019,13(4):447-454. |
| APA | Wang, Songshan,Lv, Jitao,&Zhang, Shuzhen.(2019).Discovery of CRR1-targeted copper deficiency response in Chlamydomonas reinhardtii exposed to silver nanoparticles.NANOTOXICOLOGY,13(4),447-454. |
| MLA | Wang, Songshan,et al."Discovery of CRR1-targeted copper deficiency response in Chlamydomonas reinhardtii exposed to silver nanoparticles".NANOTOXICOLOGY 13.4(2019):447-454. |
入库方式： OAI收割
来源：生态环境研究中心
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