Identification of Potential Long Noncoding RNA Biomarker of Mercury Compounds in Zebrafish Embryos
文献类型：期刊论文
| 作者 | Cao, Mengxi; Song, Fei; Yang, Xue; Peng, Lei; Cheng, Yang; Zheng, Qi; Liang, Yong; Wang, Chang |
| 刊名 | CHEMICAL RESEARCH IN TOXICOLOGY
 |
| 出版日期 | 2019-05 |
| 卷号 | 32期号:5页码:878-886 |
| ISSN号 | 0893-228X |
| 英文摘要 | Heavy metal pollution elicits severe environmental concern and health problem worldwide. Mercury is considered as a ubiquitous pollutant due to its versatile application in medicine, industry, and cosmetics. Long noncoding RNAs (lncRNAs) are transcripts greater than 200 nt without protein-encoding function. However, little is known about the mechanism of heavy metals induced noncoding RNA changes in aquatic organisms. To reveal the epigenetic mechanism of mercury toxicity in zebrafish embryos and explore novel specific mercury-toxicological biomarkers, several well-studied lncRNAs were screened by real-time PCR, and the spatial-temporal expression of lncRNAs biomarker was evaluated by in situ hybridization. The nerve systems of zebrafish embryos were evaluated by detecting locomotor behavior and the expression of neuro-genes. We identified a mercury responsive lncRNA, metastasis-associated lung adenocarcinoma transcript 1 (malat1), among five candidate lncRNAs. HgCl2, MeHg, PbCl2, CdCl2, and K2CrO4 exposure assay showed that malat-1 was a mercury specific induced lncRNAs. Malat1 was highly expressed in the brain region, eyes, and notochord of developing zebrafish embryos after exposure to mercury compounds. HgCl2 showed neurobehavior disturbance and changed neuro-genes expression pattern in zebrafish larvae. This study provides a biological method to detect inorganic or organic mercury using malat1 as a novel biomarker of mercury contamination and also clues for the exploration of neurotoxicity mechanism of mercury compounds. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42960]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Wuhan Inst Technol, Sch Environm Ecol & Biol Engn, Wuhan 430205, Hubei, Peoples R China 2.Jianghan Univ, Hubei Key Lab Environm & Hlth Effects Persistent, Wuhan 430056, Hubei, Peoples R China 3.Jianghan Univ, Inst Environm & Hlth, Wuhan 430056, Hubei, Peoples R China 4.Jianghan Univ, Inst Environm & Hlth, Minist Educ, Key Lab Optoelect Chem Mat & Devices, Wuhan 430056, Hubei, Peoples R China 5.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Mengxi,Song, Fei,Yang, Xue,et al. Identification of Potential Long Noncoding RNA Biomarker of Mercury Compounds in Zebrafish Embryos[J]. CHEMICAL RESEARCH IN TOXICOLOGY,2019,32(5):878-886. |
| APA | Cao, Mengxi.,Song, Fei.,Yang, Xue.,Peng, Lei.,Cheng, Yang.,...&Wang, Chang.(2019).Identification of Potential Long Noncoding RNA Biomarker of Mercury Compounds in Zebrafish Embryos.CHEMICAL RESEARCH IN TOXICOLOGY,32(5),878-886. |
| MLA | Cao, Mengxi,et al."Identification of Potential Long Noncoding RNA Biomarker of Mercury Compounds in Zebrafish Embryos".CHEMICAL RESEARCH IN TOXICOLOGY 32.5(2019):878-886. |
入库方式： OAI收割
来源：生态环境研究中心
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