Nucleic acid aptamers improving fluorescence anisotropy and fluorescence polarization assays for small molecules
文献类型：期刊论文
| 作者 | Zhao, Qiang; Tao, Jeffrey; Uppal, Jagdeesh S.; Peng, Hanyong; Wang, Hailin; Le, X. Chris |
| 刊名 | TRAC-TRENDS IN ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2019 |
| 卷号 | 110页码:401-409 |
| 关键词 | Adenosine triphosphate (ATP) Aflatoxin B1 Aptamer Anisotropy Cocaine DNAzyme Nanomaterials Polarization Ochratoxin A (OTA) Toxins |
| ISSN号 | 0165-9936 |
| 英文摘要 | Affinity probes, such as nucleic acid aptamers, have been combined with fluorescence anisotropy (FA)/fluorescence polarization (FP) technology for the development of a diverse range of assays. Formation of a complex between a small fluorescent molecule and its binding partner usually increases the overall size of the fluorescent molecule and decreases its rate of rotation, resulting in increases in fluorescence anisotropy/polarization. Structure- switching of the fluorescently labeled aptamers arising from target binding can also affect molecular volume, local rotation of the fluorophore, and/or fluorescence lifetime, causing changes in anisotropy/polarization. Incorporation of the unique adsorptive properties of single-stranded nucleic acid aptamers on nanomaterials, hybridization of aptamers with complementary sequences, and the amplifiable ability of nucleic acid aptamers have broadened the applications of fluorescence anisotropy assays and enhanced their sensitivity. This review focuses on nucleic acid aptamer-based fluorescence anisotropy assays for the detection of small molecules, such as therapeutic drugs, environmental contaminants, natural toxins, and metabolites. (C) 2018 Elsevier B.V. All rights reserved. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42985]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Environm Chem & Ecotoxicol, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China 2.Univ Alberta, Dept Lab Med & Pathol, Div Analyt & Environm Toxicol, Edmonton, AB T6G 2G3, Canada |
| 推荐引用方式 GB/T 7714 | Zhao, Qiang,Tao, Jeffrey,Uppal, Jagdeesh S.,et al. Nucleic acid aptamers improving fluorescence anisotropy and fluorescence polarization assays for small molecules[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY,2019,110:401-409. |
| APA | Zhao, Qiang,Tao, Jeffrey,Uppal, Jagdeesh S.,Peng, Hanyong,Wang, Hailin,&Le, X. Chris.(2019).Nucleic acid aptamers improving fluorescence anisotropy and fluorescence polarization assays for small molecules.TRAC-TRENDS IN ANALYTICAL CHEMISTRY,110,401-409. |
| MLA | Zhao, Qiang,et al."Nucleic acid aptamers improving fluorescence anisotropy and fluorescence polarization assays for small molecules".TRAC-TRENDS IN ANALYTICAL CHEMISTRY 110(2019):401-409. |
入库方式： OAI收割
来源：生态环境研究中心
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