Phosphorylation of TET2 by AMPK is indispensable in myogenic differentiation
文献类型：期刊论文
| 作者 | Zhang, Ting; Guan, Xiaowen; Choi, Un Lam; Dong, Qiang; Lam, Melody M. T.; Zeng, Jianming; Xiong, Jun; Wang, Xianju; Poon, Terence C. W.; Zhang, Hongjie |
| 刊名 | EPIGENETICS & CHROMATIN
 |
| 出版日期 | 2019-06-04 |
| 卷号 | 12页码:- |
| 关键词 | AMPK TET2 Phosphorylation Myogenesis PAX7 |
| 英文摘要 | BackgroundTET-mediated oxidation of 5-mC participates in both passive and active DNA demethylation, which exerts a significant influence on diverse biological processes. Mass spectrometry has identified multiple phosphorylation sites of TET2. However, the functions of these phosphosites and their corresponding kinases are mostly unknown.ResultsHere, we showed that AMP-activated protein kinase (AMPK) phosphorylates murine TET2 at the serine residue 97 (S97), andthe phosphorylation enhances TET2 stability through promoting its binding to 14-3-3. AMPK ablation resulted in decreased global 5-hmC levels at the myotube stages, severe differentiation defects of C2C12 cells and significantly, total loss of expression of Pax7. Genome-wide analyses revealed increased DNA methylation at genic and enhancer regions of AMPK-null myoblasts and myotubes. Using CRISPR/Cas9 technology, we showed that a novel enhancer, which is hypermethylated in AMPK-null cells, regulates Pax7 expression. The phospho-mimicking mutant, TET2-S97E, could partly rescue the differentiation defect in AMPK-ablated C2C12 cells.ConclusionsTogether, our data demonstrated that AMPK is a critical regulator of myogenesis, partly through phosphorylating TET2. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42988]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Univ Macau, Fac Hlth Sci, Canc Ctr, Taipa, Macau, Peoples R China 2.Univ Macau, Fac Hlth Sci, Ave Univ, Taipa, Macau, Peoples R China 3.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 4.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Ting,Guan, Xiaowen,Choi, Un Lam,et al. Phosphorylation of TET2 by AMPK is indispensable in myogenic differentiation[J]. EPIGENETICS & CHROMATIN,2019,12:-. |
| APA | Zhang, Ting.,Guan, Xiaowen.,Choi, Un Lam.,Dong, Qiang.,Lam, Melody M. T..,...&Li, Gang.(2019).Phosphorylation of TET2 by AMPK is indispensable in myogenic differentiation.EPIGENETICS & CHROMATIN,12,-. |
| MLA | Zhang, Ting,et al."Phosphorylation of TET2 by AMPK is indispensable in myogenic differentiation".EPIGENETICS & CHROMATIN 12(2019):-. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。