Profiling of epigenetic DNA modifications by advanced liquid chromatography-mass spectrometry technologies
文献类型：期刊论文
| 作者 | Lai, Weiyi; Mo, Jiezhen; Yin, Junfa ; Lyu, Cong; Wang, Hailin
|
| 刊名 | TRAC-TRENDS IN ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2019 |
| 卷号 | 110页码:173-182 |
| 关键词 | LC-MS/MS Epigenetics 5-hydroxymethylation DNA methylation Ultrafiltration |
| ISSN号 | 0165-9936 |
| 英文摘要 | Four DNA bases are essential biochemical elements for coding heritable life blueprint. Meanwhile, organisms utilize modifying enzymes to post chemical groups on DNA bases to regulate gene expression, chromosome structures, and transposase activity, and to defense pathogens. The researches on the biochemical modifications of DNA bases are at the cutting edge of life sciences and chemical biology. Up to date, along with fantastic progresses, liquid chromatography hyphenated with mass spectrometry (LC-MS)-based technologies become popular and powerful tools for chemical structure-dependent identification and quantification of diverse DNA modifications. Here we provided comprehensive review on LCMS-based technologies and related pretreatment technologies for profiling of epigenetic DNA modifications, mainly focusing on innovative technologies developed for desalting, DNA digestion, and ionization for mass spectrometry detection. We also summarized the epigenetic progresses, which benefited from the development of the LC-MS methodologies and technologies, on the studies of specific DNA modifications, in particular, 5-hydroxymethylcytosine and 5-formylcytosine. (C) 2018 Elsevier B.V. All rights reserved. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42995]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lai, Weiyi,Mo, Jiezhen,Yin, Junfa,et al. Profiling of epigenetic DNA modifications by advanced liquid chromatography-mass spectrometry technologies[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY,2019,110:173-182. |
| APA | Lai, Weiyi,Mo, Jiezhen,Yin, Junfa,Lyu, Cong,&Wang, Hailin.(2019).Profiling of epigenetic DNA modifications by advanced liquid chromatography-mass spectrometry technologies.TRAC-TRENDS IN ANALYTICAL CHEMISTRY,110,173-182. |
| MLA | Lai, Weiyi,et al."Profiling of epigenetic DNA modifications by advanced liquid chromatography-mass spectrometry technologies".TRAC-TRENDS IN ANALYTICAL CHEMISTRY 110(2019):173-182. |
入库方式： OAI收割
来源：生态环境研究中心
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