Silver Nanoparticles Compromise Female Embryonic Stem Cell Differentiation through Disturbing X Chromosome Inactivation
文献类型：期刊论文
| 作者 | Zhang, Jie; Chen, Yongjiu; Gao, Ming; Wang, Zhe; Liu, Rui; Xia, Tian; Liu, Sijin |
| 刊名 | ACS NANO
 |
| 出版日期 | 2019-02 |
| 卷号 | 13期号:2页码:2050-2061 |
| 关键词 | silver nanoparticles embryonic stem cells differentiation X chromosome inactivation imbalance |
| ISSN号 | 1936-0851 |
| 英文摘要 | The widespread use of silver nanoparticles (AgNPs) has raised substantial health risks to human beings. Despite a wealth of progress on toxicity studies, the understanding of the adverse effects on fetuses, embryos, and early stage cells is still rather limited, particularly under low-dose exposure settings. Moreover, nearly all previous studies ascribed AgNP-induced toxic effects to oxidative stress. Differently, we here unearthed a mechanism, namely, interruption of X chromosome inactivation (XCI) in female mouse embryonic stem cells (mESCs). Albeit with no observable cytotoxicity, significant differentiation retardation was found in female mESCs upon low-dose AgNP exposure. Mechanistic investigations uncovered expedited inactivation for the inactive X chromosome (Xi) and attenuated maintenance of the active X chromosome (Xa) state during mESC differentiation upon the challenge of low-dose AgNPs, indicative of disordered XCI. Thereby, a few X-linked genes (which are closely involved in orchestrating ESC differentiation) were found to be repressed, partially attributable to reinforced enrichment of histone modification (e.g., histone 3 lysine 27 trimethylation, H3K27me3) on their promoter regions, as the result of disordered XCI. In stark contrast to female mESCs, no impairment of differentiation was observed in male mESCs under low-dose AgNP exposure. All considered, our data unearthed that AgNPs at low concentrations compromised the differentiation program of female mESCs through disturbing XCI. Thus, this work would provide a model for the type of studies necessary to advance the understandings on AgNP-induced developmental toxicity. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/43011]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Univ Calif Los Angeles, Dept Med, Div NanoMed, Los Angeles, CA 90095 USA 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 4.Xinxiang Med Univ, Sch Publ Hlth, Xinxiang 453003, Henan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jie,Chen, Yongjiu,Gao, Ming,et al. Silver Nanoparticles Compromise Female Embryonic Stem Cell Differentiation through Disturbing X Chromosome Inactivation[J]. ACS NANO,2019,13(2):2050-2061. |
| APA | Zhang, Jie.,Chen, Yongjiu.,Gao, Ming.,Wang, Zhe.,Liu, Rui.,...&Liu, Sijin.(2019).Silver Nanoparticles Compromise Female Embryonic Stem Cell Differentiation through Disturbing X Chromosome Inactivation.ACS NANO,13(2),2050-2061. |
| MLA | Zhang, Jie,et al."Silver Nanoparticles Compromise Female Embryonic Stem Cell Differentiation through Disturbing X Chromosome Inactivation".ACS NANO 13.2(2019):2050-2061. |
入库方式： OAI收割
来源：生态环境研究中心
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