Structure-guided post-SELEX optimization of an ochratoxin A aptamer
文献类型：期刊论文
| 作者 | Xu, Guohua; Zhao, Jiajing; Liu, Na; Yang, Minghui; Zhao, Qiang; Li, Conggang; Liu, Maili |
| 刊名 | NUCLEIC ACIDS RESEARCH
 |
| 出版日期 | 2019-06-20 |
| 卷号 | 47期号:11页码:5963-5972 |
| ISSN号 | 0305-1048 |
| 英文摘要 | SELEX is the cornerstone for aptamer research with broad applications in biosensors and medicine. To improve the affinity of selected aptamers, we propose a structure-guided post-SELEX approach, an optimizationmethod based on the precise secondary structure of the aptamer-ligand complex. We demonstrate this approach using the Ochratoxin A (OTA) aptamer. Guided by the structure, we designed a new aptamer whose affinity is improved by more than 50-fold. We also determined the high-resolution NMR structure of the new aptamer-OTA complex and elucidated the discriminatory recognition mechanism of one atomic difference between two analogs, OTA and OTB. The aptamer forms an unusual hairpin structure containing an intramolecular triple helix, which is not seen in the previously determined aptamer complex. The pi-pi stacking, the hydrophobic interaction, hydrogen bonds and halogen bonds between OTA and the aptamer contribute to the recognition of OTA, and the halogen bonds play an important role in discriminating between OTA and OTB. Our results demonstrate that the structure-guided post-SELEX approach improves aptamers affinity. An improved OTA biosensor system might be developed using this new strategy. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/43024]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst,Wuhan Inst Phy, State Key Lab Magnet Resonance & Atom & Mol Phys, Natl Ctr Magnet Resonance Wuhan,Wuhan Natl Lab Op, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Guohua,Zhao, Jiajing,Liu, Na,et al. Structure-guided post-SELEX optimization of an ochratoxin A aptamer[J]. NUCLEIC ACIDS RESEARCH,2019,47(11):5963-5972. |
| APA | Xu, Guohua.,Zhao, Jiajing.,Liu, Na.,Yang, Minghui.,Zhao, Qiang.,...&Liu, Maili.(2019).Structure-guided post-SELEX optimization of an ochratoxin A aptamer.NUCLEIC ACIDS RESEARCH,47(11),5963-5972. |
| MLA | Xu, Guohua,et al."Structure-guided post-SELEX optimization of an ochratoxin A aptamer".NUCLEIC ACIDS RESEARCH 47.11(2019):5963-5972. |
入库方式： OAI收割
来源：生态环境研究中心
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