Synthesis and purification of biotinylated oligodeoxynucleotides containing single TpT dimeric pyrimidine (6-4) pyrimidone lesion
文献类型：期刊论文
| 作者 | Wu, Danni; Zhang, Ning; Kong, Bingjie; Hang, Haiying; Wang, Hailin |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2019-07 |
| 卷号 | 411期号:18页码:4123-4129 |
| 关键词 | UV irradiation Pyrimidine (6-4) pyrimidone (6-4PP) HPLC UHPLC-Q-TOF/MS |
| ISSN号 | 1618-2642 |
| 英文摘要 | Ultraviolet (UV) radiation could induce pyrimidine-related dimeric lesions in genomic DNA. Though the cyclobutane pyrimidine dimers (CPDs) are the most abundant UV-induced lesions, the pyrimidine (6-4) pyrimidone photoproducts (6-4PPs) may have more serious, potentially lethal, and mutagenic effects. It is important to have 6-4PP-containing oligodeoxynucleotides to be prepared for studying their adverse biological effects. Here, we developed a UV-irradiated water droplet method for the preparation of a biotinylated, 6-4PP-containing 10-mer oligodeoxynucleotide. By the use of HPLC purification and enrichment twice, the final yield is estimated to be about 8.1%. In contrast, without applying droplet technique, the direct UV irradiation against oligonucleotide-containing aqueous solution, the product yield is very low. The enzymatic hydrolyzation of the obtained product shows a 6-4PP characteristic ion transition of 545.12 -> 432.13 in negative ion mode UHPLC-Q-TOF/MS. The established procedure for the preparation of 6-4PP-containing oligonucleotides is convenient with an improved yield. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/43029]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Prot & Peptide Pharmaceut, Natl Lab Biomacromol, Inst Biophys, Beijing 100101, Peoples R China 2.Chinese Acad Sci, State Key Lab Environm Chem & Ecotoxicol, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Danni,Zhang, Ning,Kong, Bingjie,et al. Synthesis and purification of biotinylated oligodeoxynucleotides containing single TpT dimeric pyrimidine (6-4) pyrimidone lesion[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2019,411(18):4123-4129. |
| APA | Wu, Danni,Zhang, Ning,Kong, Bingjie,Hang, Haiying,&Wang, Hailin.(2019).Synthesis and purification of biotinylated oligodeoxynucleotides containing single TpT dimeric pyrimidine (6-4) pyrimidone lesion.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,411(18),4123-4129. |
| MLA | Wu, Danni,et al."Synthesis and purification of biotinylated oligodeoxynucleotides containing single TpT dimeric pyrimidine (6-4) pyrimidone lesion".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411.18(2019):4123-4129. |
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来源：生态环境研究中心
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