Tuning the performance of graphene as a dual-ion-mode MALDI matrix by chemical functionalization and sample incubation
文献类型：期刊论文
| 作者 | Huang, Xiu; Liu, Qian; Jiang, Guibin
|
| 刊名 | TALANTA
 |
| 出版日期 | 2019-07-01 |
| 卷号 | 199页码:532-540 |
| 关键词 | Graphene Chemical functionalization MALDI-TOF MS Sample incubation MALDI matrix Mass spectrometry |
| ISSN号 | 0039-9140 |
| 英文摘要 | Graphene-based materials have emerged as a new class of matrix for matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS), but the factors governing the performance of graphene are not fully understood. In this study, we show two independent novel approaches to tune the performance of graphene as a dual-ion-mode MALDI matrix, i.e., chemical modification of graphene (e.g., oxidation, fluorination, amination, and carboxylation) and incubation of samples with graphene. We found that both approaches could significantly affect the MS signals, enabling a deep optimization of the performance of graphene in both positive and negative ion mode MALDI-TOF MS. Results showed that the performance of graphene in MALDI was dependent on its inherent chemical properties, self-assembly behavior, and interaction with targets. The two approaches were also validated in surface-enhanced laser desorption/ionization (SELDI). Under the optimized conditions, high sensitivity and good reproducibility were obtained for targets. To our knowledge, this is also the first report that aminated and carboxylated graphene were used as MALDI matrices. This work shows novel approaches to tailor graphene for its applications, and it also helps better understand the mechanism of the LDI process. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/43047]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Jianghan Univ, Inst Environm & Hlth, Wuhan 430056, Hubei, Peoples R China 3.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Xiu,Liu, Qian,Jiang, Guibin. Tuning the performance of graphene as a dual-ion-mode MALDI matrix by chemical functionalization and sample incubation[J]. TALANTA,2019,199:532-540. |
| APA | Huang, Xiu,Liu, Qian,&Jiang, Guibin.(2019).Tuning the performance of graphene as a dual-ion-mode MALDI matrix by chemical functionalization and sample incubation.TALANTA,199,532-540. |
| MLA | Huang, Xiu,et al."Tuning the performance of graphene as a dual-ion-mode MALDI matrix by chemical functionalization and sample incubation".TALANTA 199(2019):532-540. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。