A Simple and Rapid Cryopreservation Technique for Ciliates: A Long-Term Storage Procedure Used for Marine Scuticociliates
文献类型：期刊论文
| 作者 | Liu, Yongqiang; Nan, Bei; Duan, Lili; Cheng, Ting; Bourland, William A.; Liu, Mingjian; Zhao, Yan |
| 刊名 | JOURNAL OF EUKARYOTIC MICROBIOLOGY
 |
| 出版日期 | 2019-09-01 |
| 卷号 | 66期号:5页码:836-848 |
| 关键词 | Freezing liquid nitrogen methodology protozoa recovery efficiency |
| ISSN号 | 1066-5234 |
| 英文摘要 | Pseudocohnilembus persalinus is a free-living marine scuticociliate that, as a new model organism, has been used in a wide variety of studies. However, long-term laboratory maintenance for this species is mainly achieved by subculture that requires rigorous culture environments and, too often, cultures of the organism die out for a variety of reasons. Successful transport of viable cultures also poses problems for researchers. This study describes a simple and rapid protocol for long-term cryopreservation of P. persalinus. The effects of physiological states of individuals before freezing, the type and concentration of cryoprotectant, and optimal temperatures for freezing and thawing were assessed. A cryopreservation protocol, using a mixture of 30% glycerol and 70% concentrated P. persalinus cell culture, incorporating rate-controlled freezing at -80 degrees C before liquid nitrogen storage, maintained a high recovery efficiency after 8 wk of storage. These results suggest that broader application of this protocol to build a cryopreserved marine protozoa culture bank for biological studies may be possible. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42619]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 作者单位 | 1.Boise State Univ, Dept Biol Sci, Boise, ID 83725 USA 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266003, Shandong, Peoples R China 3.Ocean Univ China, Inst Evolut & Marine Biodivers, Qingdao 266003, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yongqiang,Nan, Bei,Duan, Lili,et al. A Simple and Rapid Cryopreservation Technique for Ciliates: A Long-Term Storage Procedure Used for Marine Scuticociliates[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2019,66(5):836-848. |
| APA | Liu, Yongqiang.,Nan, Bei.,Duan, Lili.,Cheng, Ting.,Bourland, William A..,...&Zhao, Yan.(2019).A Simple and Rapid Cryopreservation Technique for Ciliates: A Long-Term Storage Procedure Used for Marine Scuticociliates.JOURNAL OF EUKARYOTIC MICROBIOLOGY,66(5),836-848. |
| MLA | Liu, Yongqiang,et al."A Simple and Rapid Cryopreservation Technique for Ciliates: A Long-Term Storage Procedure Used for Marine Scuticociliates".JOURNAL OF EUKARYOTIC MICROBIOLOGY 66.5(2019):836-848. |
入库方式： OAI收割
来源：生态环境研究中心
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