Developmental dynamics of antibiotic resistome in aerobic biofilm microbiota treating wastewater under stepwise increasing tigecycline concentrations
文献类型：期刊论文
| 作者 | Tian, Zhe; Liu, Ruyin; Zhang, Hong ; Yang, Min; Zhang, Yu
|
| 刊名 | ENVIRONMENT INTERNATIONAL
 |
| 出版日期 | 2019-10-01 |
| 卷号 | 131页码:- |
| 关键词 | Dynamics Resistome Biofilm microbiota Tigecycline Metagenomic sequencing |
| ISSN号 | 0160-4120 |
| 英文摘要 | This study aimed to investigate the impact of tigecycline, the third generation tetracycline, on the antibiotic resistance development in environmental microbiota. Two biological contact oxidation reactors containing aerobic biofilm microbiota were constructed, one of which was constantly fed with synthetic wastewater spiked with increasing concentrations of tigecycline (0 to 25 mg/L) under a hydrolytic retention time of 24 h. Over a period of 636 days, chemical oxygen demand removal over 90% and complete nitrification were achieved for both the control and tigecycline-exposed reactors, and effluent tigecycline concentrations in the tigecycline-exposed system were always < 0.051 mg/L. Significant increases (p < 0.01) in resistome abundance and resistant bacteria ratio were detected at a tigecycline dose of 10 and 25 mg/L, respectively, revealed by metagenomic sequencing and culture-based method. The increase of resistome in the tigecycline system was mainly attributed to the enrichment of tetX, one cooperative tetracycline degrading gene. Partial canonical correspondence analysis showed that the change of resistome was mainly driven by bacterial community shift (vertical pathway). Network and genome binning analyses further suggested that the proliferation of Flavobacterium harboring tetX contributed to a relatively low community-wide resistance development in the aerobic biofilm microbiota under tigecycline selection by reducing the antibiotic concentration. This work provides scientific bases for the management and evaluation of the resistance risk induced by this novel antibiotic. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/42638]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Aquat Chem, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Natl Engn Lab Ind Wastewater Treatment, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, Zhe,Liu, Ruyin,Zhang, Hong,et al. Developmental dynamics of antibiotic resistome in aerobic biofilm microbiota treating wastewater under stepwise increasing tigecycline concentrations[J]. ENVIRONMENT INTERNATIONAL,2019,131:-. |
| APA | Tian, Zhe,Liu, Ruyin,Zhang, Hong,Yang, Min,&Zhang, Yu.(2019).Developmental dynamics of antibiotic resistome in aerobic biofilm microbiota treating wastewater under stepwise increasing tigecycline concentrations.ENVIRONMENT INTERNATIONAL,131,-. |
| MLA | Tian, Zhe,et al."Developmental dynamics of antibiotic resistome in aerobic biofilm microbiota treating wastewater under stepwise increasing tigecycline concentrations".ENVIRONMENT INTERNATIONAL 131(2019):-. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。