Near-Infrared Voltage Nanosensors Enable Real-Time Imaging of Neuronal Activities in Mice and Zebrafish
文献类型：期刊论文
| 作者 | Liu, JN; Zhang, RW; Shang, CF; Zhang, Y; Feng, Y; Pan, LM; Xu, B; Hyeon, T; Bu, WB; Shi, JL |
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2020-04-29 |
| 期号 | 17页码:7858 |
| ISSN号 | 0002-7863 |
| DOI | 10.1021/jacs.0c01025 |
| 文献子类 | Article |
| 英文摘要 | Optical voltage sensors with the ability to monitor neuronal activities are invaluable tools for studying information processing of the brain. However, the current genetically encoded voltage indicators usually require high-power visible light for excitation and are limited to genetically addressable model animals. Here, we report a near-infrared (NIR)-excited nongenetic voltage nanosensor that achieves stable recording of neuronal membrane potential in intact animals. The nanosensor is composed of a Forster resonance energy transfer (FRET) pair, the outer membrane-anchored upconversion nanoparticle (UCNP), and the membrane-embedded dipicrylamine (DPA). The negative charge of DPA allows membrane potential fluctuation to affect the distance between the DPA and UCNP, therefore changing the FRET efficiency. Consequently, the emission intensity of the nanosensor can report the membrane potential. Using the nanosensor, we monitor not only electrically evoked changes in the membrane potential of cultured cells but also sensory responses of neurons in intact zebrafish and brain state-modulated subthreshold activities of cortical neurons in intact mice. |
| WOS关键词 | LUMINESCENT GOLD NANOPARTICLES ; UPCONVERTING NANOPARTICLES ; ELECTRICAL-ACTIVITY ; BRAIN-STIMULATION ; MEMBRANE ; NANOCRYSTALS ; CALCIUM |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.sic.ac.cn/handle/331005/28109]  |
| 专题 | 中国科学院上海硅酸盐研究所 |
| 推荐引用方式 GB/T 7714 | Liu, JN,Zhang, RW,Shang, CF,et al. Near-Infrared Voltage Nanosensors Enable Real-Time Imaging of Neuronal Activities in Mice and Zebrafish[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2020(17):7858. |
| APA | Liu, JN.,Zhang, RW.,Shang, CF.,Zhang, Y.,Feng, Y.,...&Du, JL.(2020).Near-Infrared Voltage Nanosensors Enable Real-Time Imaging of Neuronal Activities in Mice and Zebrafish.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY(17),7858. |
| MLA | Liu, JN,et al."Near-Infrared Voltage Nanosensors Enable Real-Time Imaging of Neuronal Activities in Mice and Zebrafish".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY .17(2020):7858. |
入库方式： OAI收割
来源：上海硅酸盐研究所
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