Macrophage polarization by plasma sprayed ceria coatings on titanium-based implants: Cerium valence state matters
文献类型：期刊论文
| 作者 | Shao, DD; Li, K; You, MY; Liu, SW; Hu, T; Huang, LP; Xie, YT; Zheng, XB |
| 刊名 | APPLIED SURFACE SCIENCE
 |
| 出版日期 | 2020-02-28 |
| ISSN号 | 0169-4332 |
| DOI | 10.1016/j.apsusc.2019.144070 |
| 文献子类 | Article |
| 英文摘要 | With inflammation increasingly recognized as a key factor that influences fracture healing, the immune response is considered as a critical component in determining biomaterial-mediated osteogenesis. Here, the ceria coatings are applied to the titanium substrates via plasma spraying under different process gas compositions, forming A-III (low Ce4+ /Ce3+ ratio) and B-IV (high Ce4+ /Ce3+ ratio) coatings. Results of protein adsorption and conformation indicated that the exposed cell-binding sites of fibronectin and subsequent cell morphology were associated with the Ce valence states. The A-III and B-IV coatings clearly restricted and promoted the spreading of the adherent RAW264.7 macrophages, respectively. Cell morphology differences between the coatings correlated with a change in the percentage of RAW cells expressing M2 surface markers Cd206/163, together with expression of anti-inflammatory cytokines Il10/1ra, while there was no significant difference between them in the percentage of Ml-type macrophages. Owing to its higher catalase-mimetic activity, the B-IV coating exerted stronger inhibitory effects on reactive oxygen species generation and Nfkb activity than either A-III coating or Ti substrate. Consistently, the lowest expression levels of Nficb-regulated pro-inflammatory cytokines 1l6 and Tnfa were found for the B-IV coating. The manipulation of cerium valence states could be a good strategy in the design of orthopedic/dental implant coatings with beneficial immune response. |
| WOS关键词 | NF-KAPPA-B ; MESENCHYMAL STEM-CELL ; OXIDE NANOPARTICLES ; MIMETIC ACTIVITY ; INFLAMMATION ; SURFACES ; CEO2 ; XPS ; ROS ; DIFFERENTIATION |
| WOS研究方向 | Chemistry ; Materials Science ; Physics |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.sic.ac.cn/handle/331005/28284]  |
| 专题 | 中国科学院上海硅酸盐研究所 |
| 推荐引用方式 GB/T 7714 | Shao, DD,Li, K,You, MY,et al. Macrophage polarization by plasma sprayed ceria coatings on titanium-based implants: Cerium valence state matters[J]. APPLIED SURFACE SCIENCE,2020. |
| APA | Shao, DD.,Li, K.,You, MY.,Liu, SW.,Hu, T.,...&Zheng, XB.(2020).Macrophage polarization by plasma sprayed ceria coatings on titanium-based implants: Cerium valence state matters.APPLIED SURFACE SCIENCE. |
| MLA | Shao, DD,et al."Macrophage polarization by plasma sprayed ceria coatings on titanium-based implants: Cerium valence state matters".APPLIED SURFACE SCIENCE (2020). |
入库方式： OAI收割
来源：上海硅酸盐研究所
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