Construction of Pepstatin A-Conjugated ultrasmall SPIONs for targeted positive MR imaging of epilepsy-overexpressed P-glycoprotein
文献类型：期刊论文
| 作者 | Du, CJ; Wang, JH; Liu, XP; Li, HM; Geng, DY; Yu, LD; Chen, Y; Zhang, J |
| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2020-02-01 |
| ISSN号 | 0142-9612 |
| DOI | 10.1016/j.biomaterials.2019.119581 |
| 文献子类 | Article |
| 英文摘要 | Surgical resection of the epileptogenic region is typically regarded to be practical and efficient for complete elimination of intractable seizures, which cannot be simply controlled by anti-epileptic drugs alone. To achieve a precision removal of the epileptogenic region and even a surgical cure, molecular imaging of epilepsy markers is highly essential for non-invasive accurate detection of the epileptogenic region. In this work, a peptide-targeted nanoprobe, based on ultrasmall superparamagnetic iron oxide nanoparticles (USPIONs), PA-USPIONs, was elaborately constructed to enable highly selective delivery and sensitive T-1-weighted positive magnetic resonance (MR) imaging of the epileptogenic region. Especially, Pepstatin A (PA), a small peptide which can specifically target to P-glycoprotein (P-gp) overexpressed at the epileptogenic region in a kainic acid (KA)-induced mice model of seizures, was conjugated onto the surface of PEGylated USPIONs. It has been demonstrated that the as-constructed PA-USPIONs nanoprobes have favorable T-1 contrast enhancement and high r(1) relaxivity compared with the clinically used T-1-MR contrast agent (Gd-DTPA) by systematic in vitro and vivo assessments. Importantly, the toxicity evaluation, especially to brains, was assessed by the histological as well as hematological examinations, demonstrating that the fabricated PA-USPIONs nanoprobes are featured with excellent biocompatibility, guaranteeing the further potential clinical application. This first report on the development of USPIONs as T-1-weighted MR contrast agents for active targeting of the epileptogenic region holds the high potential for precise resection of the according lesion in order to achieve therapeutic, often curative purposes. |
| WOS关键词 | IRON-OXIDE NANOPARTICLES ; MEDIATED MULTIDRUG-RESISTANCE ; IN-VITRO ; LOCALIZATION ; BRAIN ; TUMOR ; MONODISPERSE ; SEIZURE ; FOCUS ; PET |
| WOS研究方向 | Engineering ; Materials Science |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.sic.ac.cn/handle/331005/28346]  |
| 专题 | 中国科学院上海硅酸盐研究所 |
| 推荐引用方式 GB/T 7714 | Du, CJ,Wang, JH,Liu, XP,et al. Construction of Pepstatin A-Conjugated ultrasmall SPIONs for targeted positive MR imaging of epilepsy-overexpressed P-glycoprotein[J]. BIOMATERIALS,2020. |
| APA | Du, CJ.,Wang, JH.,Liu, XP.,Li, HM.,Geng, DY.,...&Zhang, J.(2020).Construction of Pepstatin A-Conjugated ultrasmall SPIONs for targeted positive MR imaging of epilepsy-overexpressed P-glycoprotein.BIOMATERIALS. |
| MLA | Du, CJ,et al."Construction of Pepstatin A-Conjugated ultrasmall SPIONs for targeted positive MR imaging of epilepsy-overexpressed P-glycoprotein".BIOMATERIALS (2020). |
入库方式： OAI收割
来源：上海硅酸盐研究所
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