Organic gel (OG) has excellent characteristics, including a large surface area, adjustable pore/channel size,
and good chemical stability, and has attracted great attention in the field of materials. However, the OG
packed column is difficult to pack due to the weak mechanical strength and poor monodispersity. Herein,
1-allyl-3-methyl imidazolium hexafluorophosphate-co-1-dodecanethiol ([AMIm]PF 6 -co-TDDM) was pre-
pared on the silica microsphere for chromatographic packing available in multimode liquid chromatogra-
phy (LC) mode with the good mechanical properties of silica microspheres through a simple OG synthesis
method. [AMIm]PF 6 -co-TDDM@SiO 2 hybrid microspheres with uniform particles and narrow particle size
distribution are used as stationary phases of LC. These microspheres are used in anion-exchange (IEC),
reversed-phase (RP), and hydrophilic interaction (HILIC) mode for the separation of different analytes.
Such microspheres can also be used for the preliminary qualitative analysis of active ingredients in actual
samples in addition to organic acids, alkylbenzenes, and nucleoside bases. The [AMIm]PF 6 -co-TDDM@SiO 2
chromatography packing also has good reproducibility and stability. The adhesive properties of organogels
and the adsorption properties of silica gel simplify the synthesis of stationary phase materials. This sim-
ple and effective strategy for preparing [AMIm]PF 6 -co-TDDM@SiO 2 composite microspheres by one-pot
method can expand the application of OG as a functional additive on silica microspheres in LC