Four previously undescribed steroids, identified as (3S,7S,8S,9S,10R,13S,14S,16S,17R,20S)-7 alpha-methoxy-ergosta-5,24(28)-dien-3 beta,16 beta,20-triol (1), ergosta-5,24(28)-dien-3 beta,7 alpha,16 beta-triol (2), ergosta-5,25-dien-3 beta,7 alpha,16 beta,20-tetrol (3) and 7 alpha,16 beta,24 alpha-trihydroxy-varninasterol (4), as well as five known analogues (5-9), were isolated from the leaves and twigs of Dysoxylum pallens Hiern (Meliaceae). Their structures were elucidated based on extensive spectroscopic analysis such as HR-ESI-MS, 1D and 2D NMR, UV, and IR. The absolute configuration of compound 1 was determined by X-ray diffraction analysis. Selected compounds were evaluated for their cytotoxic activities. Compounds 1, 2, and 8 exhibited moderate cytotoxic activity against HL-60, Hela, and HepG2 tumor cell lines with IC50 ranged from 11.09 to 17.51 mu M.