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Unambiguous Phosphosite Localization through the Combination of Trypsin and LysargiNase Mirror Spectra in a Large-Scale Phosphoproteome Study

文献类型：期刊论文

作者Xu, Feng3; Yu, Li1,2; Peng, Xuehui3,5; Zhang, Junling3; Li, Suzhen3,4; Liu, Shu3; Yin, Yanan5; An, Zhiwu1,2; Wang, Fuqiang3; Fu, Yan1,2
刊名JOURNAL OF PROTEOME RESEARCH
出版日期2020-06-05
卷号19期号:6页码:2185-2194
关键词trypsin LysargiNase combined spectra ion coverage phosphosite localization PTMiner
ISSN号1535-3893
DOI10.1021/acs.jproteome.9b00562
英文摘要Understanding of the kinase-guided signaling pathways requires the identification and analysis of phosphosites. Mass spectrometry (MS)-based phosphoproteomics is a rapid and highly sensitive approach for high-throughput identification of phosphosites. However, phosphosite determination from MS data with a single protease is more likely to be ambiguous, regardless of the strategy used for phosphopeptide detection. Here, we explored the application of LysargiNase, which was recently reported to mirror trypsin in specificity to cleave arginine and lysine residues exclusively at the N-terminal side. We found that the combination of trypsin and LysargiNase mirror spectra resulted in higher ion coverage in MS(2 )spectra. The median ion coverage values of b ions in tryptic spectra, LysargiNase spectra, and combined spectra are 8.3, 20.5, and 25.0%, respectively. As for the median ion coverage of y ions, these values are 27.8, 10.0, and 32.3%. Higher ion coverage was helpful to pinpoint the precise phosphosites. Compared to trypsin alone, the combined use of trypsin and LysargiNase mirror spectra enabled 67.1% of mirror spectra with unreliable scores (confidence score <0.75) to become reliable (confidence score >= 0.75). Meanwhile, all of the mirror peptide-spectrum matches (PSMs) with multiple potential phosphosites from trypsin and LysargiNase digests could be assigned one precise phosphosite after applying the combination strategy. Besides, the combination strategy could identify more novel phosphosites than the union strategy did. We synthesized three phosphopeptides corresponding to the three novel phosphosites and validated the reliability of the identification. Taken together, our data demonstrated the distinctive potential of the combination strategy presented here for unambiguous phosphosite localization (Project accession PXD011178).
资助项目MOST[2017YFC0906600] ; MOST[2016YFA0501300] ; MOST[2017YFA0505000] ; MOST[2017YFA0505100] ; National Natural Science Foundation of China[31670834] ; National Natural Science Foundation of China[31700723] ; National Natural Science Foundation of China[91839302] ; National Natural Science Foundation of China[31870824] ; Innovation Foundation of Medicine[16CXZ027] ; Innovation Foundation of Medicine[BWS17J032] ; Innovation Foundation of Medicine[BWS14J052] ; National Megaprojects for Key Infectious Diseases[2018ZX10302302001] ; Guangzhou Science and Technology Innovation & Development Project[201802020016] ; Unilevel 21st Century Toxicity Program[MA-2018-02170N] ; Foundation of State Key Lab of Proteomics[SKLP-Y201501] ; Foundation of State Key Lab of Proteomics[SKLP-K201705] ; NCMIS CAS
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000538772300002
出版者AMER CHEMICAL SOC
源URL[http://ir.amss.ac.cn/handle/2S8OKBNM/51647]  
专题应用数学研究所
通讯作者Xu, Feng; Fu, Yan; Xu, Ping
作者单位1.Univ Chinese Acad Sci, Sch Math Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Acad Math & Syst Sci, Natl Ctr Math & Interdisciplinary Sci, Key Lab Random Complex Struct & Data Sci, Beijing 100864, Peoples R China
3.Beijing Inst Life, Natl Ctr Prot Sci Beijing, Beijing Proteome Res Ctr, State Key Lab Prote, Beijing 102206, Peoples R China
4.Anhui Med Univ, Hefei 230032, Peoples R China
5.Wuhan Univ, Sch Pharmaceut Sci, Key Lab Combinatorial Biosynth & Drug Discovery, Sch Med,Minist Educ, Wuhan 430072, Peoples R China
推荐引用方式
GB/T 7714		 Xu, Feng,Yu, Li,Peng, Xuehui,et al. Unambiguous Phosphosite Localization through the Combination of Trypsin and LysargiNase Mirror Spectra in a Large-Scale Phosphoproteome Study[J]. JOURNAL OF PROTEOME RESEARCH,2020,19(6):2185-2194.
APA Xu, Feng.,Yu, Li.,Peng, Xuehui.,Zhang, Junling.,Li, Suzhen.,...&Xu, Ping.(2020).Unambiguous Phosphosite Localization through the Combination of Trypsin and LysargiNase Mirror Spectra in a Large-Scale Phosphoproteome Study.JOURNAL OF PROTEOME RESEARCH,19(6),2185-2194.
MLA Xu, Feng,et al."Unambiguous Phosphosite Localization through the Combination of Trypsin and LysargiNase Mirror Spectra in a Large-Scale Phosphoproteome Study".JOURNAL OF PROTEOME RESEARCH 19.6(2020):2185-2194.

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来源：数学与系统科学研究院

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