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Revelation of AbfR in regulation of mismatch repair and energy metabolism in S. epidermidis by integrated proteomic and metabolomic analysis

文献类型：期刊论文

作者Gu, Lei1,2,3; Liu, Xing2,3; Wang, Yu-Qiu1; Zhou, Yan-ting2,3; Zhu, Hong-wen2,3; Huang, Jin1; Lan, Le-fu4,5; Zheng, Jing1; Yang, Cai-guang4,5; Zhou, Hu2,3,5
刊名JOURNAL OF PROTEOMICS
出版日期2020-08-30
卷号226页码:10
关键词Staphylococcus epidermidis AbfR transcription factor Oxidation-sensing regulator Oxidative stress TMT10-plex labelling Antibiotic resistance regulator
ISSN号1874-3919
DOI10.1016/j.jprot.2020.103900
通讯作者Zheng, Jing(zhengjing@ecust.edu.cn) ; Yang, Cai-guang(yangcg@simm.ac.cn) ; Zhou, Hu(zhouhu@simm.ac.cn)
英文摘要Staphylococcus epidermidis is a common causative of nosocomial infections associated with indwelling medical devices. To date, the mechanisms of the pathogenicity and drug resistance of S. epidermidis have not been clearly elucidated. AbfR has been previously identified as an oxidation-sensing regulator that regulates bacterial aggregation and biofilm formation by responding to oxidative stress in S. epidermidis; however, the regulatory pathways of AbfR are underexplored. In this study, we investigated the oxidation-sensing regulatory mechanism of AbfR using TMT10-plex labelling quantitative proteomic and untargeted metabolomic approaches. Integrated analysis of two omics datasets indicated that abfR depletion influenced nucleic acid metabolism and activated the DNA mismatch repair pathway. In addition, several energy-related metabolic pathways, including tricarboxylic acid (TCA) cycle, glycolysis, and arginine metabolism, were remarkably impacted by the deletion of abfR. This study revealed the regulatory networks of the transcription factor AbfR from a multi-omics view and demonstrated that AbfR played a broad role in not only mismatch repair but also energy metabolism, enabling S. epidermidis to constantly sense and adapt to environmental stress. Significance: Staphylococcus epidermidis has emerged as a major nosocomial infection causing pathogen. AbfR, a transcription factor of S. epidermidis, plays an important role in oxidative stress, cell aggregation, and biofilm formation; however, the regulatory mechanism of AbfR is unknown. Using proteomic and metabolomic approaches, this study unveils the global regulatory networks of AbfR, and demonstrates that AbfR not only regulates the DNA mismatch repair pathway by an oxidation sensing mechanism but also affects energy metabolism. This study expands the body of knowledge related to regulatory transcription factors in staphylococci and lays a foundation for future research on clinical infections caused by S. epidermidis.
WOS关键词STAPHYLOCOCCUS-AUREUS ; MARR FAMILY ; ARGININE ; BINDING
资助项目Fundamental Research Funds for the Central Universities ; Science and Technology Commission Shanghai Municipality[17XD1404400] ; National Natural Science Foundation of China[81138046] ; Open Project Program of State Key Laboratory of Innovative Natural Medicine and TCM Injections[QFSKL2017005] ; Nova Development Program of the Shanghai Institute of Materia Medica ; [31500110]
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000566934000005
出版者ELSEVIER
源URL[http://119.78.100.183/handle/2S10ELR8/291112]  
专题新药研究国家重点实验室
通讯作者Zheng, Jing; Yang, Cai-guang; Zhou, Hu
作者单位1.East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, Shanghai 201203, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
5.Univ Chinese Acad Sci, Number 19A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Gu, Lei,Liu, Xing,Wang, Yu-Qiu,et al. Revelation of AbfR in regulation of mismatch repair and energy metabolism in S. epidermidis by integrated proteomic and metabolomic analysis[J]. JOURNAL OF PROTEOMICS,2020,226:10.
APA Gu, Lei.,Liu, Xing.,Wang, Yu-Qiu.,Zhou, Yan-ting.,Zhu, Hong-wen.,...&Zhou, Hu.(2020).Revelation of AbfR in regulation of mismatch repair and energy metabolism in S. epidermidis by integrated proteomic and metabolomic analysis.JOURNAL OF PROTEOMICS,226,10.
MLA Gu, Lei,et al."Revelation of AbfR in regulation of mismatch repair and energy metabolism in S. epidermidis by integrated proteomic and metabolomic analysis".JOURNAL OF PROTEOMICS 226(2020):10.

入库方式： OAI收割

来源：上海药物研究所

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