Low-protein/high-carbohydrate diet induces AMPK-dependent canonical and non-canonical thermogenesis in subcutaneous adipose tissue
文献类型：期刊论文
| 作者 | Aquilano, Katia1; Sciarretta, Francesca5; Turchi, Riccardo1; Li, Bo-Han2; Rosina, Marco1; Ceci, Veronica1; Guidobaldi, Giulio1; Arena, Simona3; D'Ambrosio, Chiara3; Audano, Matteo4 |
| 刊名 | REDOX BIOLOGY
 |
| 出版日期 | 2020-09-01 |
| 卷号 | 36页码:14 |
| ISSN号 | 2213-2317 |
| 关键词 | Systems physiology Mitochondria Metabolism Serca Ucp1 |
| DOI | 10.1016/j.redox.2020.101633 |
| 通讯作者 | Aquilano, Katia(katia.aquilano@uniroma2.it) ; Lettieri-Barbato, Daniele(daniele.lettieri.barbato@uniroma2.it) |
| 英文摘要 | Low-protein/high-carbohydrate (LPHC) diet has been suggested to promote metabolic health and longevity in adult humans and animal models. However, the complex molecular underpinnings of how LPHC diet leads to metabolic benefits remain elusive. Through a multi-layered approach, here we observed that LPHC diet promotes an energy-dissipating response consisting in the parallel recruitment of canonical and non-canonical (muscular) thermogenic systems in subcutaneous white adipose tissue (sWAT). In particular, we measured Ucp1 induction in association with up-regulation of actomyosin components and several Serca (Serca1, Serca2a, Serca2b) ATPases. In beige adipocytes, we observed that AMPK activation is responsible for transducing the amino acid lowering in an enhanced fat catabolism, which sustains both Ucp1-and Serca-dependent energy dissipation. Limiting AMPK activation counteracts the expression of brown fat and muscular genes, including Ucp1 and Serca, as well as mitochondrial oxidative genes. We observed that mitochondrial reactive oxygen species are the upstream molecules controlling AMPK-mediated metabolic rewiring in amino acid-restricted beige adipocytes. Our findings delineate a novel metabolic phenotype of responses to amino acid shortage, which recapitulates some of the benefits of cool temperature in sWAT. In conclusion, this highlights LPHC diet as a valuable and practicable strategy to prevent metabolic diseases through the enhancement of mitochondrial oxidative metabolism and the recruitment of different energy dissipating routes in beige adipocytes. |
| WOS关键词 | SKELETAL-MUSCLE ; CALORIC RESTRICTION ; ENERGY-EXPENDITURE ; BROWN ; MACRONUTRIENTS ; SARCOLIPIN ; ADIPOCYTES ; REGULATOR ; DISTINCT ; OBESITY |
| 资助项目 | European Foundation for the Study of Diabetes (EFSD/Lilly) ; Italian Ministry of Health[GR-2018-12367588] ; University of Rome Tor Vergata (Uncovering Excellence Program) ; AIRC[IG23562] ; National Natural Science Foundation of China[81470166] ; MIUR Progetto Eccellenza ; Italian Ministry of Health ; CNR project NUTRAGE[DSB.AD004.271] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000571102000002 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/291231]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Aquilano, Katia; Lettieri-Barbato, Daniele |
| 作者单位 | 1.Univ Roma Tor Vergata, Dept Biol, Via Ric Sci, Rome, Italy 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 3.CNR, Prote & Mass Spectrometry Lab, ISPAAM, Naples, Italy 4.Univ Milan, Dept Pharmacol & Biomol Sci, Milan, Italy 5.IRCCS Fdn Santa Lucia, Rome, Italy 6.Univ Molise, Dept Biosci & Terr, Pesche, IS, Italy 7.Univ Naples Federico II, Dept Mol Med & Med Biotechnol, Naples, Italy 8.Fdn IRCCS Casa Sollievo Sofferenza Hosp, Gastroenterol Unit, San Giovanni Rotondo, FG, Italy |
| 推荐引用方式 GB/T 7714 | Aquilano, Katia,Sciarretta, Francesca,Turchi, Riccardo,et al. Low-protein/high-carbohydrate diet induces AMPK-dependent canonical and non-canonical thermogenesis in subcutaneous adipose tissue[J]. REDOX BIOLOGY,2020,36:14. |
| APA | Aquilano, Katia.,Sciarretta, Francesca.,Turchi, Riccardo.,Li, Bo-Han.,Rosina, Marco.,...&Lettieri-Barbato, Daniele.(2020).Low-protein/high-carbohydrate diet induces AMPK-dependent canonical and non-canonical thermogenesis in subcutaneous adipose tissue.REDOX BIOLOGY,36,14. |
| MLA | Aquilano, Katia,et al."Low-protein/high-carbohydrate diet induces AMPK-dependent canonical and non-canonical thermogenesis in subcutaneous adipose tissue".REDOX BIOLOGY 36(2020):14. |
入库方式： OAI收割
来源：上海药物研究所
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