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The impact of structural modification of sulfated polysaccharides on bone morphogenic protein 2 and inhibition of endothelial cell angiogenesis

文献类型：期刊论文

作者Wang, Ying1,2; Xiao, Fei1,2; Jin, Can1,2; Wang, Wucheng1,2; Chen, Xia1,2; Liu, Qin1,2; Ding, Kan1,2
刊名CARBOHYDRATE RESEARCH
出版日期2020-10-01
卷号496页码:10
关键词Sulfated polysaccharides Bone morphogenic protein 2 Structure and activity relationship Angiogenesis
ISSN号0008-6215
DOI10.1016/j.carres.2020.108093
通讯作者Ding, Kan(dingkan@simm.ac.cn)
英文摘要Sulfated polysaccharides play important roles in angiogenesis. However, the impact of structural alteration of sulfated polysaccharide on the bioactivity is still vague. In this study, binding between different sulfated polysaccharides and bone morphogenic protein 2 (BMP2) was measured to understand the sense of this motif transformation. The results showed that binding between sulfated alpha-1,4-glucan and BMP2 was the most intensive. The branch of alpha-1,4-glucan was important for the binding. The affinity of sulfated polysaccharides to BMP2 increased as the molecular weight (MW) and degree of substitution (DS) increased. DS that exceeded 1.05 impaired binding and played more important role in polysaccharide BMP2 interaction than MW. The reservation of partial 6-OH would benefit its binding ability to BMP2. Further, we showed that sulfated polysaccharides with strong binding to BMP2 blocked phosphorylation of Smad 1/5/8 and expression of Id1 to a greater extent than those not strongly bind to BMP2. The binding strength of polysaccharides to BMP2 increased, so did the potency of the anti-angiogenesis effects.
WOS关键词DISRUPTING ANGIOGENESIS ; TARGETING VEGFR2/VEGF ; GROWTH ; DERIVATIVES ; EXPRESSION ; PROSTATE ; HEPARIN
资助项目Key New Drug Creation and Manufacturing Program, Ministry of Science and Technology[2019ZX09735001] ; National Key R&D Program of China, Ministry of Science and Technology[2019YFC1711000] ; key program of National Natural Science Foundation of China[31230022]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000571835100004
出版者ELSEVIER SCI LTD
源URL[http://119.78.100.183/handle/2S10ELR8/291262]  
专题中国科学院上海药物研究所
通讯作者Ding, Kan
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Key Lab Receptor Res, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China
2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Ying,Xiao, Fei,Jin, Can,et al. The impact of structural modification of sulfated polysaccharides on bone morphogenic protein 2 and inhibition of endothelial cell angiogenesis[J]. CARBOHYDRATE RESEARCH,2020,496:10.
APA Wang, Ying.,Xiao, Fei.,Jin, Can.,Wang, Wucheng.,Chen, Xia.,...&Ding, Kan.(2020).The impact of structural modification of sulfated polysaccharides on bone morphogenic protein 2 and inhibition of endothelial cell angiogenesis.CARBOHYDRATE RESEARCH,496,10.
MLA Wang, Ying,et al."The impact of structural modification of sulfated polysaccharides on bone morphogenic protein 2 and inhibition of endothelial cell angiogenesis".CARBOHYDRATE RESEARCH 496(2020):10.

入库方式： OAI收割

来源：上海药物研究所

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