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The unprecedented diversity of UGT94-family UDP-glycosyltransferases in Panax plants and their contribution to ginsenoside biosynthesis

文献类型：期刊论文

作者Yang, Chengshuai1,2; Li, Chaojing1,4; Wei, Wei1; Wei, Yongjun1; Liu, Qunfang3; Zhao, Guoping1,2; Yue, Jianmin3; Yan, Xing1; Wang, Pingping1; Zhou, Zhihua1
刊名SCIENTIFIC REPORTS
出版日期2020-09-21
卷号10期号:1页码:11
ISSN号2045-2322
DOI10.1038/s41598-020-72278-y
通讯作者Yan, Xing(yanxing@sibs.ac.cn) ; Wang, Pingping(ppwang@sibs.ac.cn) ; Zhou, Zhihua(zhouzhihua@sippe.ac.cn)
英文摘要More than 150 ginsenosides have been isolated and identified from Panax plants. Ginsenosides with different glycosylation degrees have demonstrated different chemical properties and bioactivity. In this study, we systematically cloned and characterized 46 UGT94 family UDP-glycosyltransferases (UGT94s) from a mixed Panax ginseng/callus cDNA sample with high amino acid identity. These UGT94s were found to catalyze sugar chain elongation at C3-O-Glc and/or C20-O-Glc of protopanaxadiol (PPD)-type, C20-O-Glc or C6-O-Glc of protopanaxatriol (PPT)-type or both C3-O-Glc of PPD-type and C6-O-Glc of PPT-type or C20-O-Glc of PPD-type and PPT-type ginsenosides with different efficiencies. We also cloned 26 and 51 UGT94s from individual P. ginseng and P. notoginseng plants, respectively; our characterization results suggest that there is a group of UGT94s with high amino acid identity but diverse functions or catalyzing activities even within individual plants. These UGT94s were classified into three clades of the phylogenetic tree and consistent with their catalytic function. Based on these UGT94s, we elucidated the biosynthetic pathway of a group of ginsenosides. Our present results reveal a series of UGTs involved in second sugar chain elongation of saponins in Panax plants, and provide a scientific basis for understanding the diverse evolution mechanisms of UGT94s among plants.
WOS关键词SEQUENTIAL GLUCOSYLATION ; SAPONINS
资助项目National Key Research and Development Program of China[2018YFA0900700] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB27020206] ; Key Deployment Projects of the Chinese Academy of Sciences[KFZD-SW-215] ; Strategic Biological Resources Service Network Plan of the Chinese Academy of Sciences[KFJ-BRP-009] ; International Great Science Program of the Chinese Academy of Sciences[153D31KYSB20170121] ; Science and Technology Commission of Shanghai Municipality[17YF1422500]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000573765900008
出版者NATURE RESEARCH
源URL[http://119.78.100.183/handle/2S10ELR8/291302]  
专题新药研究国家重点实验室
通讯作者Yan, Xing; Wang, Pingping; Zhou, Zhihua
作者单位1.Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, CAS Key Lab Synthet Biol, Shanghai 200032, Peoples R China
2.Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Synthet Biol, Shenzhen 518055, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Chengshuai,Li, Chaojing,Wei, Wei,et al. The unprecedented diversity of UGT94-family UDP-glycosyltransferases in Panax plants and their contribution to ginsenoside biosynthesis[J]. SCIENTIFIC REPORTS,2020,10(1):11.
APA Yang, Chengshuai.,Li, Chaojing.,Wei, Wei.,Wei, Yongjun.,Liu, Qunfang.,...&Zhou, Zhihua.(2020).The unprecedented diversity of UGT94-family UDP-glycosyltransferases in Panax plants and their contribution to ginsenoside biosynthesis.SCIENTIFIC REPORTS,10(1),11.
MLA Yang, Chengshuai,et al."The unprecedented diversity of UGT94-family UDP-glycosyltransferases in Panax plants and their contribution to ginsenoside biosynthesis".SCIENTIFIC REPORTS 10.1(2020):11.

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来源：上海药物研究所

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