A pectin-like polysaccharide from Polygala tenuifolia inhibits pancreatic cancer cell growth in vitro and in vivo by inducing apoptosis and suppressing autophagy
文献类型：期刊论文
| 作者 | Bian, Youcheng1,2,3; Zeng, Hui1,3; Tao, Hong1,3; Huang, Lulin1,3; Du, Zhenyun1,3; Wang, Jiao2; Ding, Kan1,3
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| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| 出版日期 | 2020-11-01 |
| 卷号 | 162页码:107-115 |
| ISSN号 | 0141-8130 |
| 关键词 | Polygala tenuifolia Pectin Pancreatic cancer Apoptosis Autophagy |
| DOI | 10.1016/j.ijbiomac.2020.06.054 |
| 通讯作者 | Ding, Kan(dingkan@simm.ac.cn) |
| 英文摘要 | Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal malignant carcinomas, which is characterized by apoptosisand autophagy-dependent tumorigenic growth. Autophagy constitutes a stress adaptation that suppresses apoptosis. To explore new leading compound against PDAC, a pectin-like polysaccharide named RP02-1, was purified from roots of Polygala tenuifolia. Bioactivity test showed that RP02-1 might inhibit pancreatic cancer cells growth in vitro and in vivo. RP02-1 could inhibit pancreatic cancer cell (AsPC-1 and BxPC-3) proliferation, migration and colony formation. Mechanism study suggested that RP02-1 induced pancreatic cancer cells apoptosis, which was detected by Bcl-2 down-regulation, Bax up-regulation and conversion from Caspase 3 to Cleaved Caspase 3. Interestingly, autophagy was suppressed by RP02-1 treatment concentration dependently through affenuatingBeclin-1, ATG5 and LC3B expression in BxPC-3 cells. In addition, RP02-1 could inhibit autophagy induced by Pennogenin 3-O-beta-chacotrioside. However, RP02-1 had almost no toxicity both in vitro and in vivo. The above results suggested that RP02-1 might be a potential leading compound for new drug candidate development for human PDAC treatment via inducing apoptosis and against autophagy. (c) 2020 Elsevier B.V. All rights reserved. |
| WOS关键词 | GALECTIN-3 INHIBITOR ; GCS-100 |
| 资助项目 | Key New Drug Creation and Manufacturing Program[2019ZX09735001] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020373] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000577936100011 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/291501]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Ding, Kan |
| 作者单位 | 1.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 2.Shanghai Univ, Sch Life Sci, 333 Nanchen Rd, Shanghai 200444, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Key Lab Receptor Res, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bian, Youcheng,Zeng, Hui,Tao, Hong,et al. A pectin-like polysaccharide from Polygala tenuifolia inhibits pancreatic cancer cell growth in vitro and in vivo by inducing apoptosis and suppressing autophagy[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,162:107-115. |
| APA | Bian, Youcheng.,Zeng, Hui.,Tao, Hong.,Huang, Lulin.,Du, Zhenyun.,...&Ding, Kan.(2020).A pectin-like polysaccharide from Polygala tenuifolia inhibits pancreatic cancer cell growth in vitro and in vivo by inducing apoptosis and suppressing autophagy.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,162,107-115. |
| MLA | Bian, Youcheng,et al."A pectin-like polysaccharide from Polygala tenuifolia inhibits pancreatic cancer cell growth in vitro and in vivo by inducing apoptosis and suppressing autophagy".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 162(2020):107-115. |
入库方式： OAI收割
来源：上海药物研究所
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