Standardization and harmonization of distributed multi-center proteotype analysis supporting precision medicine studies
文献类型：期刊论文
| 作者 | Xuan, Yue13,14,15,16; Bateman, Nicholas W.14,15; Gallien, Sebastien1,16; Goetze, Sandra17,18; Zhou, Yue19; Navarro, Pedro13; Hu, Mo19; Parikh, Niyati14,15; Hood, Brian L.14,15; Conrads, Kelly A.14,15 |
| 刊名 | NATURE COMMUNICATIONS
 |
| 出版日期 | 2020-10-16 |
| 卷号 | 11期号:1页码:12 |
| ISSN号 | 2041-1723 |
| DOI | 10.1038/s41467-020-18904-9 |
| 通讯作者 | Xuan, Yue(yue.xuan@thermofisher.com) ; Wollscheid, Bernd(wbernd@ethz.ch) ; Conrads, Thomas P.(conrads@whirc.org) |
| 英文摘要 | Cancer has no borders: Generation and analysis of molecular data across multiple centers worldwide is necessary to gain statistically significant clinical insights for the benefit of patients. Here we conceived and standardized a proteotype data generation and analysis workflow enabling distributed data generation and evaluated the quantitative data generated across laboratories of the international Cancer Moonshot consortium. Using harmonized mass spectrometry (MS) instrument platforms and standardized data acquisition procedures, we demonstrate robust, sensitive, and reproducible data generation across eleven international sites on seven consecutive days in a 24/7 operation mode. The data presented from the high-resolution MS1-based quantitative data-independent acquisition (HRMS1-DIA) workflow shows that coordinated proteotype data acquisition is feasible from clinical specimens using such standardized strategies. This work paves the way for the distributed multi-omic digitization of large clinical specimen cohorts across multiple sites as a prerequisite for turning molecular precision medicine into reality. Distributed multi-omic digitization of clinical specimen across multiple sites is a prerequisite for turning molecular precision medicine into reality. Here, the authors show that coordinated proteotype data acquisition is feasible using standardized MS data acquisition and analysis strategies. |
| WOS关键词 | DATA-INDEPENDENT ACQUISITION ; RETENTION TIME ; PEPTIDE ; IDENTIFICATION ; THROUGHPUT ; PROTEOMICS ; TOOLS |
| 资助项目 | Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen ; Wissenschaft und Forschung ; Bundesministerium fur Bildung und Forschung ; National Cancer Institute Early Detection Research Network[1U01CA214194-01] ; CIHR[PJT 156357] ; Collaborative Personalized Cancer Medicine Team Grant from the Princess Margaret Cancer Centre ; Congressionally Directed Medical Research Program's Ovarian Cancer Research Program[W81XWH19-1-0183] ; Congressionally Directed Medical Research Program's Ovarian Cancer Research Program[W81XWH-16-2-0038] ; Uniformed Services University of the Health Sciences (USUHS)[HU0001-16-2-0006] ; Uniformed Services University of the Health Sciences (USUHS)[HU0001-16-2-0014] ; Uniformed Services University of the Health Sciences (USUHS)[HU0001-18-1-0012] ; Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc. ; Academia Sinica ; Ministry of Science and Technology in Taiwan ; VILLUM Center for Bioanalytical Sciences (VILLUM Foundation)[7292] ; VILLUM Center for Bioanalytical Sciences (VILLUM Foundation)[507200007B] ; National Key R&D Program of China[2017YFC0908403] ; Cancer Center Amsterdam and Netherlands Organization for Scientific Research (NWO) ; Australian NHMRC Early Career fellowship ; Personalized Health and Related Technologies (PHRT) strategic focus area of ETH |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | NATURE RESEARCH |
| WOS记录号 | WOS:000582056600011 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/291507]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Xuan, Yue; Wollscheid, Bernd; Conrads, Thomas P. |
| 作者单位 | 1.Thermo Fisher Sci, Precis Med Sci Ctr, Cambridge, MA USA 2.Chinese Acad Sci, Shanghai Inst Mat Med, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China 3.BGI SHENZHEN, Beishan Rd, Shenzhen 518083, Guangdong, Peoples R China 4.Univ Southern Denmark, Dept Biochem & Mol Biol, Campusvej 55, DK-5230 Odense M, Denmark 5.Princess Margaret Canc Ctr, 101 Coll St PMCRT 9-807, Toronto, ON M5G 1L7, Canada 6.Natl Translat Sci Ctr Mol Med, Xian 710032, Peoples R China 7.Air Force Med Univ, Sch Basic Med, Dept Cell Biol, Xian 710032, Peoples R China 8.Univ Sydney, Sydney Mass Spectrometry, Sydney, NSW 2006, Australia 9.Ruhr Univ Bochum, Med Proteom Ctr MPC, Med Fak, D-44801 Bochum, Germany 10.Univ Aberdeen, Coll Phys Sci, Dept Chem, Aberdeen AB243FX, Scotland |
| 推荐引用方式 GB/T 7714 | Xuan, Yue,Bateman, Nicholas W.,Gallien, Sebastien,et al. Standardization and harmonization of distributed multi-center proteotype analysis supporting precision medicine studies[J]. NATURE COMMUNICATIONS,2020,11(1):12. |
| APA | Xuan, Yue.,Bateman, Nicholas W..,Gallien, Sebastien.,Goetze, Sandra.,Zhou, Yue.,...&Conrads, Thomas P..(2020).Standardization and harmonization of distributed multi-center proteotype analysis supporting precision medicine studies.NATURE COMMUNICATIONS,11(1),12. |
| MLA | Xuan, Yue,et al."Standardization and harmonization of distributed multi-center proteotype analysis supporting precision medicine studies".NATURE COMMUNICATIONS 11.1(2020):12. |
入库方式： OAI收割
来源：上海药物研究所
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