Systematic comparison of metabolic differences of Uncaria rhynchophylla in rat, mouse, dog, pig, monkey and human liver microsomes
文献类型：期刊论文
| 作者 | Li, Hao-Jv1,2; Wei, Wen-Long1; Li, Zhen-Wei1,2; Yao, Chang-Liang1; Wang, Meng-Yuan1,2; Zhang, Jian-Qing1; Li, Jia-Yuan1; Guo, De-An1,2
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| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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| 出版日期 | 2020-09-05 |
| 页码 | 7 |
| 关键词 | Uncaria rhynchophylla Indole alkaloids Mass defect filter Time-staggered targeted ion lists Metabolite identification Liver microsomes |
| ISSN号 | 1618-2642 |
| DOI | 10.1007/s00216-020-02922-z |
| 通讯作者 | Guo, De-An(daguo@simm.ac.cn) |
| 英文摘要 | Metabolites have a close relationship with the efficacy and safety of herbal medicines. However, ubiquitous matrix interferences, complex co-elution, and minor or trace amounts in plasma restrict the comprehensive identification of metabolites. In this study, an efficient strategy comprising a mass defect filter and time-staggered targeted ion lists was established to characterize the metabolites of alkaloids ofUncaria rhynchophylla(UR) for the systematic comparison of metabolic differences in rat, mouse, dog, pig, monkey and human liver microsomes. The mass defect filter model effectively decreased interfering ions by 63-68%, and time-staggered precursor ion lists significantly increased the number of triggered MS/MS fragmentation by 65-120% in liver microsomes of six species. Ultimately, a total of 165 metabolites in the liver microsomes of six species were tentatively characterized, and the main metabolic pathways were demethylation, isomerization, hydrolysis, oxygenation and dehydrogenation. The results showed that the mouse liver microsomes exhibited metabolic behavior most similar to human metabolism of UR alkaloids. We hope that these results provide basic data for further investigation of UR metabolism in different species, and that the strategy can provide a reference for metabolite characterization of herbal medicines in complex biological matrix. |
| WOS关键词 | INTEGRATED STRATEGY ; LC-MS/MS ; ALKALOIDS ; ISORHYNCHOPHYLLINE ; PHARMACOKINETICS ; CLASSIFICATION ; IDENTIFICATION ; PHYTOCHEMISTRY ; ISOCORYNOXEINE ; PHARMACOLOGY |
| 资助项目 | National Key R&D Program of China[2019YFC1711000] ; National Natural Science Foundation of China[81530095] ; National Natural Science Foundation of China[81673591] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020348] ; National Standardization of Traditional Chinese Medicine Project[ZYBZH K LN 01] ; Science and Technology Commission Foundation of Shanghai[15DZ0502800] ; Guangdong Provincial Key R&D Plan in key priority fields[2020B1111110007] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000566049300001 |
| 出版者 | SPRINGER HEIDELBERG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/291586]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Guo, De-An |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Haike Rd 501, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Hao-Jv,Wei, Wen-Long,Li, Zhen-Wei,et al. Systematic comparison of metabolic differences of Uncaria rhynchophylla in rat, mouse, dog, pig, monkey and human liver microsomes[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2020:7. |
| APA | Li, Hao-Jv.,Wei, Wen-Long.,Li, Zhen-Wei.,Yao, Chang-Liang.,Wang, Meng-Yuan.,...&Guo, De-An.(2020).Systematic comparison of metabolic differences of Uncaria rhynchophylla in rat, mouse, dog, pig, monkey and human liver microsomes.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,7. |
| MLA | Li, Hao-Jv,et al."Systematic comparison of metabolic differences of Uncaria rhynchophylla in rat, mouse, dog, pig, monkey and human liver microsomes".ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2020):7. |
入库方式： OAI收割
来源：上海药物研究所
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