Structure of formylpeptide receptor 2-G(i) complex reveals insights into ligand recognition and signaling
文献类型：期刊论文
| 作者 | Zhuang, Youwen2,3,4; Liu, Heng5; Zhou, X. Edward4; Verma, Ravi Kumar6; de Waal, Parker W.4; Jang, Wonjo7; Xu, Ting-Hai4; Wang, Lei5; Meng, Xing8; Zhao, Gongpu8 |
| 刊名 | NATURE COMMUNICATIONS
 |
| 出版日期 | 2020-02-14 |
| 卷号 | 11期号:1页码:12 |
| ISSN号 | 2041-1723 |
| DOI | 10.1038/s41467-020-14728-9 |
| 通讯作者 | Xu, H. Eric(Eric.Xu@simm.ac.cn) ; Zhang, Cheng(chengzh@pitt.edu) |
| 英文摘要 | Formylpeptide receptors (FPRs) as G protein-coupled receptors (GPCRs) can recognize formylpeptides derived from pathogens or host cells to function in host defense and cell clearance. In addition, FPRs, especially FPR2, can also recognize other ligands with a large chemical diversity generated at different stages of inflammation to either promote or resolve inflammation in order to maintain a balanced inflammatory response. The mechanism underlying promiscuous ligand recognition and activation of FPRs is not clear. Here we report a cryo-EM structure of FPR2-G(i) signaling complex with a peptide agonist. The structure reveals a widely open extracellular region with an amphiphilic environment for ligand binding. Together with computational docking and simulation, the structure suggests a molecular basis for the recognition of formylpeptides and a potential mechanism of receptor activation, and reveals conserved and divergent features in G(i) coupling. Our results provide a basis for understanding the molecular mechanism of the functional promiscuity of FPRs. |
| WOS关键词 | FORMYL-PEPTIDE RECEPTOR ; PROTEIN-COUPLED RECEPTORS ; VAL-D-MET ; ACTIVATES NEUTROPHILS ; PATTERN-RECOGNITION ; AGONIST ; IDENTIFICATION ; INFLAMMATION ; RESOLUTION ; DATABASE |
| 资助项目 | National Institutes of Health grants[1R35GM128641] ; National Institutes of Health grants[1R01GM127710] ; National Institutes of Health grants[1R01GM130142] ; Van Andel Research Institute[XDB08020303] ; Biomedical Research Council of A*STAR including the Industry Alignment Fund Pre-Positioning[H18/01/a0/C14] ; UCAS joint Ph.D. Training Program |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000564260600002 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/291595]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Xu, H. Eric; Zhang, Cheng |
| 作者单位 | 1.Takeda Res, 9625 Towne Ctr Dr, San Diego, CA 92130 USA 2.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Van Andel Res Inst, Program Struct Biol, Ctr Canc & Cell Biol, Grand Rapids, MI 49503 USA 5.Univ Pittsburgh, Sch Med, Dept Pharmacol & Chem Biol, Lab GPCR Biol, Pittsburgh, PA 15261 USA 6.ASTAR, Bioinformat Inst BII, Singapore, Singapore 7.Augusta Univ, Med Coll Georgia, Dept Pharmacol & Toxicol, Augusta, GA 30912 USA 8.Van Andel Res Inst, David Van Andel Adv Cryoelectron Microscopy Suite, Grand Rapids, MI 49503 USA |
| 推荐引用方式 GB/T 7714 | Zhuang, Youwen,Liu, Heng,Zhou, X. Edward,et al. Structure of formylpeptide receptor 2-G(i) complex reveals insights into ligand recognition and signaling[J]. NATURE COMMUNICATIONS,2020,11(1):12. |
| APA | Zhuang, Youwen.,Liu, Heng.,Zhou, X. Edward.,Verma, Ravi Kumar.,de Waal, Parker W..,...&Zhang, Cheng.(2020).Structure of formylpeptide receptor 2-G(i) complex reveals insights into ligand recognition and signaling.NATURE COMMUNICATIONS,11(1),12. |
| MLA | Zhuang, Youwen,et al."Structure of formylpeptide receptor 2-G(i) complex reveals insights into ligand recognition and signaling".NATURE COMMUNICATIONS 11.1(2020):12. |
入库方式： OAI收割
来源：上海药物研究所
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