Imidazole-fused benzothiadiazole-based red-emissive fluorescence probe for lysosomal pH imaging in living cells
文献类型：期刊论文
| 作者 | Li, Lingling1; Li, Yang3 ; Dang, Yijing1; Chen, Tingting3; Zhang, Ao2,3; Ding, Chunyong2,3; Xu, Zhiai1
|
| 刊名 | TALANTA
 |
| 出版日期 | 2020-09-01 |
| 卷号 | 217页码:10 |
| 关键词 | Imidazole-fused benzothiadiazole pH imaging Lysosome Fluorescence probe |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2020.121066 |
| 通讯作者 | Ding, Chunyong(chding@simm.ac.cn) ; Xu, Zhiai(zaxu@chem.ecnu.edu.cn) |
| 英文摘要 | Intracellular pH is a key physiological factor for controlling the activities and functions of cells and lysosome is a vital subcellular organelle. Thus, developing a novel lysosome-targeting fluorescence probe for selective and sensitive detection of lysosomal pH in living cells is very important. In this work, we synthesized a series of fluorescence probes based on imidazole-fused benzothiadiazole. The optical properties of these probes were easily adjusted by modifying the substituents with different electron-withdrawing/donating ability in imidazole moiety. All of them showed acid-response and decreased fluorescence intensity during pH values changing from 4.0 to 8.5. The introduction of morpholine group allowed them to specifically respond to the changes of lysosomal pH. Among them, probe MIBTAA possessed a suitable pK(a) value (5.3) and showed good linear response to pH (R-2 = 0.9918) with red emission when pH changed from 4.4 to 5.6. The probe was successfully applied for monitoring pH variation in living cells induced by proton-pump inhibitor Baf-A1 and chloroquine, indicating its great potential for pH imaging in biological applications. |
| WOS关键词 | INTRACELLULAR PH ; SILICA NANOPARTICLES |
| 资助项目 | National Natural Science Foundation of China[21675055] ; National Natural Science Foundation of China[21877120] ; National Science & Technology Major Project Key New Drug Creation and Manufacturing Program, China[2018ZX09711002] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020320] ; State Key Laboratory of Drug Research, China[SIMM1903KF-13] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000537880200059 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/291644]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Ding, Chunyong; Xu, Zhiai |
| 作者单位 | 1.East China Normal Univ, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Lingling,Li, Yang,Dang, Yijing,et al. Imidazole-fused benzothiadiazole-based red-emissive fluorescence probe for lysosomal pH imaging in living cells[J]. TALANTA,2020,217:10. |
| APA | Li, Lingling.,Li, Yang.,Dang, Yijing.,Chen, Tingting.,Zhang, Ao.,...&Xu, Zhiai.(2020).Imidazole-fused benzothiadiazole-based red-emissive fluorescence probe for lysosomal pH imaging in living cells.TALANTA,217,10. |
| MLA | Li, Lingling,et al."Imidazole-fused benzothiadiazole-based red-emissive fluorescence probe for lysosomal pH imaging in living cells".TALANTA 217(2020):10. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。