Whole-genome sequence of Phellinus gilvus (mulberry Sanghuang) reveals its unique medicinal values
文献类型：期刊论文
| 作者 | Huo, Jinxi2; Zhong, Shi2; Du, Xin2; Cao, Yinglong3,4; Wang, Wenqiong3,4; Sun, Yuqing2; Tian, Yu1; Zhu, Jianxun2; Chen, Jine2; Xuan, Lijiang3,4
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| 刊名 | JOURNAL OF ADVANCED RESEARCH
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| 出版日期 | 2020-07-01 |
| 卷号 | 24页码:325-335 |
| 关键词 | Anti-carcinogenesis activity Genome sequencing HR-ESI-MS Inoscavin A Phellinus gilvus Phenylpropanoids biosynthesis |
| ISSN号 | 2090-1232 |
| DOI | 10.1016/j.jare.2020.04.011 |
| 通讯作者 | Wu, Chongming() ; Li, Yougui() |
| 英文摘要 | Phellinus gilvus (Schwein.) Pat, a species of 'Sanghuang', has been well-documented for various medicinal uses, but the genome information and active constituents are largely unknown. Here, we sequenced the whole-genome of P. gilvus, identified phenylpropanoids as its key anti-cancer components, and deduced their biosynthesis pathways. A 41.11-Mb genome sequence was assembled and the heatmap created with high-throughput chromosome conformation capture techniques data suggested all bins could be clearly divided into 11 pseudochromosomes. Cellular experiments showed that P. gilvus fruiting body was more effective to inhibit hepatocellular carcinoma cells than mycelia. High resolution electrospray ionization mass spectroscopy (HR-ESI-MS) analysis revealed P. gilvus fruiting body was rich in phenylpropanoids, and several unique phenylpropanoids in Phellinus spp. exhibited potent anti-carcinogenesis activity. Based on genomic, HR-ESI-MS information and differentially expressed genes in transcriptome analysis, we deduced the biosynthesis pathway of four major phenylpropanoids in P. gilvus. Transcriptome analysis revealed the deduced genes expressions were synergistically changed with the production of phenylpropanoids. The optimal candidate genes of phenylpropanoids' synthesis pathway were screened by molecular docking analysis. Overall, our results provided a high-quality genomic data of P. gilvus and inferred biosynthesis pathways of four phenylpropanoids with potent anti-carcinogenesis activities. These will be a valuable resource for further genetic improvement and effective use of the P. gilvus. (C) 2020 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| WOS关键词 | HI-C ; HISPIDIN BIOSYNTHESIS ; IDENTIFICATION ; DATABASE ; INONOTUS ; FAMILIES ; CLASSIFICATION ; INHIBITION ; ASSEMBLIES ; ALIGNMENT |
| 资助项目 | Science and Technology Department of Zhejiang Province[2018C02003] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000550190000003 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/291986]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Wu, Chongming; Li, Yougui |
| 作者单位 | 1.Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev, Pharmacol & Toxicol Res Ctr, Beijing 100193, Peoples R China 2.Zhejiang Acad Agr Sci, Sericultural Res Inst, Hangzhou 310021, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huo, Jinxi,Zhong, Shi,Du, Xin,et al. Whole-genome sequence of Phellinus gilvus (mulberry Sanghuang) reveals its unique medicinal values[J]. JOURNAL OF ADVANCED RESEARCH,2020,24:325-335. |
| APA | Huo, Jinxi.,Zhong, Shi.,Du, Xin.,Cao, Yinglong.,Wang, Wenqiong.,...&Li, Yougui.(2020).Whole-genome sequence of Phellinus gilvus (mulberry Sanghuang) reveals its unique medicinal values.JOURNAL OF ADVANCED RESEARCH,24,325-335. |
| MLA | Huo, Jinxi,et al."Whole-genome sequence of Phellinus gilvus (mulberry Sanghuang) reveals its unique medicinal values".JOURNAL OF ADVANCED RESEARCH 24(2020):325-335. |
入库方式： OAI收割
来源：上海药物研究所
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