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Pao Pereira extract suppresses benign prostatic hyperplasia by inhibiting inflammation-associated NF kappa B signaling

文献类型：期刊论文

作者Dong, Yu2,3; Liu, Jiakuan4; Xue, Zesheng4; Sun, Jingya3; Huang, Zhengnan5; Jing, Yifeng5; Han, Bangmin5; Shen, Bing5; Yan, Jun1,6; Huang, Ruimin3,7
刊名BMC COMPLEMENTARY MEDICINE AND THERAPIES
出版日期2020-05-16
卷号20期号:1页码:10
关键词Pao Pereira extract BPH NF kappa B Inflammation Extracellular matrix
DOI10.1186/s12906-020-02943-2
通讯作者Shen, Bing(shenbingyishi@163.com) ; Yan, Jun(yan_jun@fudan.edu.cn) ; Huang, Ruimin(rmhuang@simm.ac.cn)
英文摘要Background: Our previous study revealed the extract from the bark of an Amazonian tree Pao Pereira can suppress benign prostatic hyperplasia (BPH) in a rat model. Herein, we examined its inhibitory effects on human BPH cells and dissect its molecular mechanism. Methods: We applied Pao extract to human BPH epithelial BPH-1 and prostate myofibroblast WPMY-1 cells. Cell viability, apoptosis and immunoblotting were performed, followed by gene expression profiling and gene set enrichment analysis (GSEA) to detect the differentially expressed genes and signaling pathway induced by Pao extract. Human ex vivo BPH explant organ culture was also used to examine the effects of Pao extract on human BPH tissues. Results: Pao extract treatment inhibited viability and induced apoptosis in human BPH-1 and WPMY-1 cells. Gene expression profiling and the following validation indicated that the expression levels of pro-apoptotic genes (eg. PCDC4, CHOP and FBXO32) were induced by Pao extract in both two cell lines. GSEA further revealed that Pao extract treatment was negatively associated with the activation of NF kappa B signaling. Pao extract suppressed the transcriptional activity of NF.B and down-regulated its target genes involved in inflammation (CXCL5, CXCL6 and CXCL12) and extracellular matrix (ECM) remodeling (HAS2, TNC and MMP13) in both cultured cells and human ex vivo BPH explants. Conclusion: In both BPH epithelial and stromal cells, Pao extract induces apoptosis by upregulating the proapoptotic genes and inhibiting the inflammation-associated NF kappa B signaling via reducing phosphorylation of NF kappa B subunit RelA. Our data suggest that Pao extract may be a promising phytotherapeutic agent for BPH.
WOS关键词HUMAN GLIOBLASTOMA CELLS ; TENASCIN-C ; EXPRESSION ; PROLIFERATION ; CANCER ; GENE ; INVASION ; GROWTH
资助项目National Natural Science Foundation[81672873] ; National Natural Science Foundation[81872373] ; National Science & Technology Major Project Key New Drug Creation and Manufacturing Program, China[2018ZX09711002] ; One Hundred Talent Program of Chinese Academy of Sciences ; Wu Jieping Medical Foundation[320.6750.16051] ; Shanghai Songjiang Municipal Science and Technology Commission Natural Science Foundation[17SJKJGG10] ; Shanghai Specialized Research Fund for Integrated Chinese and Western Medicine in General Hospitals[ZHYYZXYJHZX-1-201705] ; Beljanski Foundation
WOS研究方向Integrative & Complementary Medicine
语种英语
WOS记录号WOS:000550358200002
出版者BMC
源URL[http://119.78.100.183/handle/2S10ELR8/292060]  
专题中国科学院上海药物研究所
通讯作者Shen, Bing; Yan, Jun; Huang, Ruimin
作者单位1.Nanjing Univ, Model Anim Res Ctr, MOE Key Lab Model Anim Dis Study, Nanjing, Jiangsu, Peoples R China
2.Shanghai Univ, Shanghai, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China
4.Nanjing Univ, Model Anim Res Ctr, Nanjing, Jiangsu, Peoples R China
5.Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Urol, 100 Haining Rd, Shanghai 200080, Peoples R China
6.Fudan Univ, Dept Lab Anim Sci, 130 Dongan Rd, Shanghai 200032, Peoples R China
7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Dong, Yu,Liu, Jiakuan,Xue, Zesheng,et al. Pao Pereira extract suppresses benign prostatic hyperplasia by inhibiting inflammation-associated NF kappa B signaling[J]. BMC COMPLEMENTARY MEDICINE AND THERAPIES,2020,20(1):10.
APA Dong, Yu.,Liu, Jiakuan.,Xue, Zesheng.,Sun, Jingya.,Huang, Zhengnan.,...&Huang, Ruimin.(2020).Pao Pereira extract suppresses benign prostatic hyperplasia by inhibiting inflammation-associated NF kappa B signaling.BMC COMPLEMENTARY MEDICINE AND THERAPIES,20(1),10.
MLA Dong, Yu,et al."Pao Pereira extract suppresses benign prostatic hyperplasia by inhibiting inflammation-associated NF kappa B signaling".BMC COMPLEMENTARY MEDICINE AND THERAPIES 20.1(2020):10.

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来源：上海药物研究所

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