中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
Liquid chromatography-tandem mass spectrometric assay for TPN171 in human plasma

文献类型：期刊论文

作者Zhang, Mengqi1,2; Liu, Haiming1,2; He, Jiajin1,2; Gui, Yuzhou1,2; Qian, Hongjie1,2; Jia, Jingying1,2; Yu, Chen1,2; Wang, Zhen3; Shen, Jing Shan3; Liu, Gangyi1,2
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
出版日期2020-11-30
卷号191页码:8
关键词TPN171 LC-MS/MS Human plasma Protein precipitation
ISSN号0731-7085
DOI10.1016/j.jpba.2020.113634
通讯作者Liu, Gangyi(gyliu@shxh-centerlab.com)
英文摘要A simple, rapid and accurate method for quantitative analysis of a highly selective phosphodiesterase-5 inhibitor (PDE5), TPN171 by high performance liquid chromatography and tandem mass spectrometry in human plasma was proposed and validated successfully using D-3-TPN171 as internal standards (ISTD). An aliquot of 100 mu L of plasma was mixed with internal standard and was precipitated with acetonitrile. Gradient elution was performed on a ACQUITY HSS T3 column (50 x 2.1 mm, 1.8 pin) coupled with a ACQUITY column in-line filter at 40 degrees C, by 5 mM ammonium acetate in water containing 0.1 % formic acid and 0.1 % formic acid in acetonitrile as the mobile phase. The total analytical run time was 3.5 min. The analyte was monitored using multiple reaction monitoring (MRM) scan in positive polarity mode. The ion transition was m/z 442.2 -> 113.2 and 445.2 -> 116.2 for TPN171 and D-3-TPN171 respectively. The method was validated for specificity, sensitivity, precision, accuracy, and other analytical parameters. The results found were satisfactory over the linear calibration range of 1-500 ng/mL. Within-day precisions ranged from 1.8 to 7.3 %, and between-day precisions from 2.3 to 4.9 %, accuracies were 95.5-99.8 %.The validated method was successfully applied to determine the plasma concentration after oral administration of 10 mg TPN171 in six healthy volunteers. (C) 2020 Elsevier B.V. All rights reserved.
WOS关键词SELECTIVE PDE5 INHIBITOR ; TADALAFIL ; HYPERTENSION ; SILDENAFIL ; DISCOVERY
WOS研究方向Chemistry ; Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000579528000060
出版者ELSEVIER
源URL[http://119.78.100.183/handle/2S10ELR8/292608]  
专题中国科学院上海药物研究所
通讯作者Liu, Gangyi
作者单位1.Fudan Univ, Zhongshan Xuhui Hosp, Shanghai Xuhui Cent Hosp, Shanghai 200031, Peoples R China
2.Shanghai Engn Res Ctr Phase I Clin Res & Qual Con, Shanghai 200031, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Mengqi,Liu, Haiming,He, Jiajin,et al. Liquid chromatography-tandem mass spectrometric assay for TPN171 in human plasma[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2020,191:8.
APA Zhang, Mengqi.,Liu, Haiming.,He, Jiajin.,Gui, Yuzhou.,Qian, Hongjie.,...&Liu, Gangyi.(2020).Liquid chromatography-tandem mass spectrometric assay for TPN171 in human plasma.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,191,8.
MLA Zhang, Mengqi,et al."Liquid chromatography-tandem mass spectrometric assay for TPN171 in human plasma".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 191(2020):8.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。