Berberine and its structural analogs have differing effects on functional profiles of individual gut microbiomes
文献类型：期刊论文
| 作者 | Li, Leyuan2; Chang, Lu1; Zhang, Xu2; Ning, Zhibin2; Mayne, Janice2; Ye, Yang3,4,5 ; Stintzi, Alain2,5; Liu, Jia1,5; Figeys, Daniel2,5,6
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| 刊名 | GUT MICROBES
 |
| 出版日期 | 2020 |
| 卷号 | 11期号:5页码:1348-1361 |
| 关键词 | Gut microbiome functionality butyrate Akkermansia berberine metaproteomics |
| ISSN号 | 1949-0976 |
| DOI | 10.1080/19490976.2020.1755413 |
| 通讯作者 | Liu, Jia(jia.liu@simm.ac.cn) |
| 英文摘要 | The understanding of the effects of compounds on the gut microbiome is limited. In particular, it is unclear whether structurally similar compounds would have similar or distinct effects on the gut microbiome. Here, we selected berberine (BBR), an isoquinoline quaternary alkaloid, and 16 structural analogs and evaluated their effects on seven individual gut microbiomes cultured in vitro. The responses of the individual microbiomes were evaluated by metaproteomic profiles and by assessing butyrate production. We show that both interindividual differences and compound treatments significantly contributed to the variance of metaproteomic profiles. BBR and eight analogs led to changes in proteins involved in microbial defense and stress responses and enrichment of proteins from Verrucomicrobia, Proteobacteria, and Bacteroidetes phyla. It also led to a decrease in proteins from the Firmicutes phylum and its Clostridiales order which correlated to decrease proteins involved in the butyrate production pathway and butyrate concentration. Three of the compounds, sanguinarine, chelerythrine, and ethoxysanguinarine, activated bacterial protective mechanisms, enriched Proteobacteria, increased opacity proteins, and markedly reduced butyrate production. Dihydroberberine had a similar function to BBR in enriching the Akkermansia genus. In addition, it showed less overall adverse impacts on the functionality of the gut microbiome, including a better maintenance of the butyrate level. Our study shows that ex vivo microbiome assay can assess differential regulating effects of compounds with subtle differences and reveals that compound analogs can have distinct effects on the microbiome. |
| WOS关键词 | IN-VITRO ; DIET ; AKKERMANSIA ; METAPROTEOMICS ; DERIVATIVES ; SIMILARITY ; INHIBITORS ; PROTEINS ; BUTYRATE ; DRUGS |
| WOS研究方向 | Gastroenterology & Hepatology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000590235500015 |
| 出版者 | TAYLOR & FRANCIS INC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/292634]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Liu, Jia |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 2.Univ Ottawa, Ottawa Inst Syst Biol, Dept Biochem Microbiol & Immunol, Fac Med, Ottawa, ON, Canada 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Nat Prod Chem Dept, Shanghai, Peoples R China 5.Univ Ottawa Joint Res Ctr Syst & Personalized Pha, Shanghai Inst Mat Med, Shanghai, Peoples R China 6.Canadian Inst Adv Res, Toronto, ON, Canada |
| 推荐引用方式 GB/T 7714 | Li, Leyuan,Chang, Lu,Zhang, Xu,et al. Berberine and its structural analogs have differing effects on functional profiles of individual gut microbiomes[J]. GUT MICROBES,2020,11(5):1348-1361. |
| APA | Li, Leyuan.,Chang, Lu.,Zhang, Xu.,Ning, Zhibin.,Mayne, Janice.,...&Figeys, Daniel.(2020).Berberine and its structural analogs have differing effects on functional profiles of individual gut microbiomes.GUT MICROBES,11(5),1348-1361. |
| MLA | Li, Leyuan,et al."Berberine and its structural analogs have differing effects on functional profiles of individual gut microbiomes".GUT MICROBES 11.5(2020):1348-1361. |
入库方式： OAI收割
来源：上海药物研究所
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