Transcriptional and proteomic insights into the host response in fatal COVID-19 cases
文献类型：期刊论文
| 作者 | Wu, Meng; Chen, Yaobing14; Xia, Han12,13; Wang, Changli11; Tan, Chin Yee10; Cai, Xunhui9; Liu, Yufeng8,9; Ji, Fenghu8,9; Xiong, Peng8,9; Liu, Ran |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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| 出版日期 | 2020-11-10 |
| 卷号 | 117期号:45页码:28336-28343 |
| ISSN号 | 0027-8424 |
| 关键词 | COVID-19 SARS-CoV-2 neutrophil fibrosis NETosis |
| DOI | 10.1073/pnas.2018030117 |
| 通讯作者 | Liu, Liang(liuliang@mail.tjmu.du.cn) ; Wang, Guoping(wanggp@hust.edu.cn) ; Surana, Neeraj K.(neil.surana@duke.edu) ; Xia, Tian(tianxia@hust.edu.cn) ; Kasper, Dennis L.(dennis_kasper@hms.harvard.edu) |
| 英文摘要 | Coronavirus disease 2019 (COVID-19), the global pandemic caused by SARS-CoV-2, has resulted thus far in greater than 933,000 deaths worldwide; yet disease pathogenesis remains unclear. Clinical and immunological features of patients with COVID-19 have highlighted a potential role for changes in immune activity in regulating disease severity. However, little is known about the responses in human lung tissue, the primary site of infection. Here we show that pathways related to neutrophil activation and pulmonary fibrosis are among the major up-regulated transcriptional signatures in lung tissue obtained from patients who died of COVID-19 in Wuhan, China. Strikingly, the viral burden was low in all samples, which suggests that the patient deaths may be related to the host response rather than an active fulminant infection. Examination of the colonic transcriptome of these patients suggested that SARS-CoV-2 impacted host responses even at a site with no obvious pathogenesis. Further proteomics analysis validated our transcriptome findings and identified several key proteins, such as the SARS-CoV-2 entry-associated protease cathepsins B and L and the inflammatory response modulator S100A8/A9, that are highly expressed in fatal cases, revealing potential drug targets for COVID-19. |
| WOS关键词 | NEUTROPHIL EXTRACELLULAR TRAPS ; READ ALIGNMENT ; LUNG |
| 资助项目 | Clinical Foundation of Tongji Hospital[XXGZBDYJ010] ; Ministry of Science and Technology of P. R. China Plan[2020YFC0844700] ; National Natural Science Foundation of China[61571202] ; NIH/National Institute of Allergy and Infectious Diseases[R01AI148273] ; National University of Singapore Development Grant |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | NATL ACAD SCIENCES |
| WOS记录号 | WOS:000590745300016 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/292660]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Liu, Liang; Wang, Guoping; Surana, Neeraj K.; Xia, Tian; Kasper, Dennis L. |
| 作者单位 | 1.Harvard Med Sch, Blavatnik Inst, Dept Immunol, Boston, MA 02115 USA 2.Duke Univ, Dept Immunol, Sch Med, Durham, NC 27710 USA 3.Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Forens Med, Wuhan 430030, Peoples R China 4.Cleveland Clin, Dept Cardiovasc & Metab Sci, Lerner Res Inst, Cleveland, OH 44195 USA 5.Baylor Coll Med, Dept Med, Houston, TX 77030 USA 6.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, Shanghai 201203, Peoples R China 7.Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Pathol, Wuhan 430030, Peoples R China 8.Huazhong Univ Sci & Technol, Inst Artificial Intelligence, Wuhan 430074, Peoples R China 9.Duke Univ, Dept Mol Genet & Microbiol, Sch Med, Durham, NC 27710 USA 10.Duke Univ, Dept Pediat, Sch Med, Durham, NC 27710 USA |
| 推荐引用方式 GB/T 7714 | Wu, Meng,Chen, Yaobing,Xia, Han,et al. Transcriptional and proteomic insights into the host response in fatal COVID-19 cases[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2020,117(45):28336-28343. |
| APA | Wu, Meng.,Chen, Yaobing.,Xia, Han.,Wang, Changli.,Tan, Chin Yee.,...&Kasper, Dennis L..(2020).Transcriptional and proteomic insights into the host response in fatal COVID-19 cases.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,117(45),28336-28343. |
| MLA | Wu, Meng,et al."Transcriptional and proteomic insights into the host response in fatal COVID-19 cases".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 117.45(2020):28336-28343. |
入库方式： OAI收割
来源：上海药物研究所
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