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Mechanism and optimization of supramolecular complexation-enhanced fluorescence spectroscopy for the determination of SN-38 in plasma and cells

文献类型：期刊论文

作者Zhao, Kena4,5; Guo, Tao4; Sun, Xian4; Xiong, Ting3,4; Ren, Xiaohong4; Wu, Li4; Yang, Rui1,2; Sun, Huimin1,2; Shi, Senlin5; Zhang, Jiwen1,3,4,5
刊名LUMINESCENCE
出版日期2020-11-19
页码12
关键词cyclodextrins inclusion complex pharmacokinetics SN‐ 38 spectrofluorimetric transfer
ISSN号1522-7235
DOI10.1002/bio.3973
通讯作者Sun, Huimin(sunhm@126.com) ; Shi, Senlin(pjstone@163.com) ; Zhang, Jiwen(jwzhang@simm.ac.cn)
英文摘要Quantitative detection of two different forms of SN-38 in biological samples is, currently, cumbersome and difficult. A revisit to the mechanism of supramolecular complexation-enhanced fluorescence spectroscopy helps to optimize the determination of SN-38 in plasma and the cellular pharmacokinetics in A549 cells based on the supramolecular complexation. Firstly, the inclusion mechanism dominated by thermodynamic constants was determined by measuring kinetic/thermodynamic parameters (k(on), k(off), Delta G, Delta H, Delta S). On this basis, the best effect of fluorescence sensitization was optimized through screening the interaction conditions (cyclodextrin species and concentrations, drug levels, temperature, pH of the buffer, and reaction time). Furthermore, the proportional relationship between the concentration of the inclusion complex and the fluorescence intensity was confirmed. Finally, a highly sensitive, selective spectrofluorimetric method was established and validated for quantitative analysis of the lactone and carboxylate molecular states of SN-38 plasma levels in rats and cell membrane transfer kinetics in A549 cell lines. The limits of detection for the lactone and carboxylate forms in plasma were found to be 0.44 ng center dot ml(-1) and 0.28 ng center dot ml(-1), respectively. Precision and accuracy met the requirements of biological samples analysis. The proposed detection method provided a reference for elucidating the biodistribution of SN-38.
WOS关键词LIPOSOME-BASED FORMULATION ; SPECTROFLUOROMETRIC DETERMINATION ; BETA-CYCLODEXTRIN ; LIQUID-CHROMATOGRAPHY ; PHARMACEUTICAL-PREPARATIONS ; INCLUSION COMPLEX ; IN-VITRO ; DELIVERY ; SN38 ; DERIVATIVES
资助项目Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12050307] ; National Science and Technology Major Projects for 'Major New Drugs Innovation and Development'[2018ZX09721002-009] ; Key Project of Chinese Ministry of Education[211072]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000590185200001
出版者WILEY
源URL[http://119.78.100.183/handle/2S10ELR8/292688]  
专题新药研究国家重点实验室
通讯作者Sun, Huimin; Shi, Senlin; Zhang, Jiwen
作者单位1.Natl Inst Food & Drug Control, NMPA Key Lab Qual Res & Evaluat Pharmaceut Excipi, Beijing 102600, Peoples R China
2.Natl Inst Food & Drug Control, Inst Control Pharmaceut Excipient & Packaging Mat, Beijing 100050, Peoples R China
3.Jiangxi Univ Tradit Chinese Med, Minist Educ, Key Lab Modern Chinese Med Preparat, Nanchang 330004, Jiangxi, Peoples R China
4.Chinese Acad Sci, Ctr Drug Delivery Syst, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
5.Zhejiang Chinese Med Univ, Coll Pharmaceut Sci, Hangzhou 311402, Peoples R China
推荐引用方式
GB/T 7714		 Zhao, Kena,Guo, Tao,Sun, Xian,et al. Mechanism and optimization of supramolecular complexation-enhanced fluorescence spectroscopy for the determination of SN-38 in plasma and cells[J]. LUMINESCENCE,2020:12.
APA Zhao, Kena.,Guo, Tao.,Sun, Xian.,Xiong, Ting.,Ren, Xiaohong.,...&Zhang, Jiwen.(2020).Mechanism and optimization of supramolecular complexation-enhanced fluorescence spectroscopy for the determination of SN-38 in plasma and cells.LUMINESCENCE,12.
MLA Zhao, Kena,et al."Mechanism and optimization of supramolecular complexation-enhanced fluorescence spectroscopy for the determination of SN-38 in plasma and cells".LUMINESCENCE (2020):12.

入库方式： OAI收割

来源：上海药物研究所

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