Determination of ginsenoside compound K in human plasma by liquid chromatography–tandem mass spectrometry of lithium adducts
文献类型：期刊论文
| 作者 | Chen Yunhui2; Lu Youming1; Yang Yong1; Chen Xiaoyan1 ; Zhu Liang2; Zhong Dafang1
|
| 刊名 | Acta Pharmaceutica Sinica B
 |
| 出版日期 | 2015 |
| 卷号 | 5期号:5页码:461 |
| 关键词 | LC?MS/MS Ginsenoside compound K Lithium adduct ion Pharmacokinetics Therapeutics. Pharmacology RM1-950 |
| ISSN号 | 2211-3835 |
| 英文摘要 | Ginsenoside compound K (GCK), the main metabolite of protopanaxadiol constituents of Panax ginseng, easily produces alkali metal adduct ions during mass spectrometry particularly with lithium. Accordingly, we have developed a rapid and sensitive liquid chromatography–tandem mass spectrometric method for analysis of GCK in human plasma based on formation of a lithium adduct. The analyte and paclitaxel (internal standard) were extracted from 50μL human plasma using methyl tert-butyl ether. Chromatographic separation was performed on a Phenomenex Gemini C18 column (50mm×2.0mm; 5μm) using stepwise gradient elution with acetonitrile–water and 0.2mmol/L lithium carbonate at a flow rate of 0.5mL/min. Detection was performed in the positive ion mode using multiple reaction monitoring of the transitions at m/z 629→449 for the GCK-lithium adduct and m/z 860→292 for the adduct of paclitaxel. The assay was linear in the concentration range 1.00–1000ng/mL (r2>0.9988) with intra- and inter-day precision of ±8.4% and accuracy in the range of ?4.8% to 6.5%. Recovery, stability and matrix effects were all satisfactory. The method was successfully applied to a pharmacokinetic study involving administration of a single GCK 50mg tablet to healthy Chinese volunteers. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/292752]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.中国科学院上海药物研究所 2.Guangdong College of Pharmacy |
| 推荐引用方式 GB/T 7714 | Chen Yunhui,Lu Youming,Yang Yong,et al. Determination of ginsenoside compound K in human plasma by liquid chromatography–tandem mass spectrometry of lithium adducts[J]. Acta Pharmaceutica Sinica B,2015,5(5):461. |
| APA | Chen Yunhui,Lu Youming,Yang Yong,Chen Xiaoyan,Zhu Liang,&Zhong Dafang.(2015).Determination of ginsenoside compound K in human plasma by liquid chromatography–tandem mass spectrometry of lithium adducts.Acta Pharmaceutica Sinica B,5(5),461. |
| MLA | Chen Yunhui,et al."Determination of ginsenoside compound K in human plasma by liquid chromatography–tandem mass spectrometry of lithium adducts".Acta Pharmaceutica Sinica B 5.5(2015):461. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。