GTS40, an active fraction of Gou Teng-San (GTS), protects PC12 from H2O2-induced cell injury through antioxidative properties
文献类型：期刊论文
| 作者 | Chen Lei3; Wei MengLin3; Zhao JiaoJiao2; Hong Hao1; Qu Wei2; Feng Feng2; Liu WenYuan3 |
| 刊名 | CHINESE JOURNAL OF NATURAL MEDICINES
 |
| 出版日期 | 2017 |
| 卷号 | 15期号:7页码:495 |
| 关键词 | BCL-2 FAMILY-MEMBERS OXIDATIVE STRESS APOPTOSIS MITOCHONDRIA ALKALOIDS DEATH MICE MS INHIBITION CHOTOSAN GTS40 Oxidative stress Neuroprotective effects Apoptosis HPLC-QTOF-MS/MS |
| ISSN号 | 2095-6975 |
| 英文摘要 | Oxidative stress, a predominant cause of apoptosis cascades triggered in neurodegenerative disorders, has been regarded as a critical inducement in the pathogenesis of Alzheimer's disease (AD). Gou Teng-San (GTS) is a traditional Chinese herbs preparation commonly utilized to alleviate cognitive dysfunction and psychological symptoms of patients with dementia. The present study aimed to investigate the protective effects of GTS40, an active fraction of GTS, on H2O2-induced oxidative damage and identify the potential active ingredients. Our results revealed that GTS40 exhibited radical scavenging activity, elevated cell viability, decreased the levels of intracellular reactive oxygen species (ROS), and stabilized mitochondrial transmembrane potential (MMP) in H2O2-treated PC12 cells. In addition, GTS40 blocked the apoptotic cascade by reversing the imbalance of Bcl-2/Bax and inhibiting the activity of caspase-3. Furthermore, an HPLC-QTOFMS method was developed to characterize major chemical constituents in GTS40. Our results revealed twenty-seven identified or tentatively characterized compounds through comparing their retention time (t(R)) and MS spectra with reference standards. These results suggested that GTS40 was a promising active fraction that may be beneficial in the prevention and treatment of oxidative stress-mediated neurodegenerative disorders. |
| 资助项目 | [National Natural Science Foundation of China] |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/292785]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.安徽肥东花生原种场 2.中国科学院上海药物研究所 3.中国科学院南京地理与湖泊研究所 |
| 推荐引用方式 GB/T 7714 | Chen Lei,Wei MengLin,Zhao JiaoJiao,et al. GTS40, an active fraction of Gou Teng-San (GTS), protects PC12 from H2O2-induced cell injury through antioxidative properties[J]. CHINESE JOURNAL OF NATURAL MEDICINES,2017,15(7):495. |
| APA | Chen Lei.,Wei MengLin.,Zhao JiaoJiao.,Hong Hao.,Qu Wei.,...&Liu WenYuan.(2017).GTS40, an active fraction of Gou Teng-San (GTS), protects PC12 from H2O2-induced cell injury through antioxidative properties.CHINESE JOURNAL OF NATURAL MEDICINES,15(7),495. |
| MLA | Chen Lei,et al."GTS40, an active fraction of Gou Teng-San (GTS), protects PC12 from H2O2-induced cell injury through antioxidative properties".CHINESE JOURNAL OF NATURAL MEDICINES 15.7(2017):495. |
入库方式： OAI收割
来源：上海药物研究所
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