The complex structures of ALKBH2 mutants cross-linked to dsDNA reveal the conformational swing of β-hairpin
文献类型：期刊论文
| 作者 | Chen Baoen2; Gan Jianhua1; Yang Caiguang2
|
| 刊名 | Science China. Chemistry
 |
| 出版日期 | 2014 |
| 卷号 | 57期号:2页码:307 |
| 关键词 | DNA repair ALKBH2 chemical cross-linking β-hairpin motif damage detection |
| ISSN号 | 1674-7291 |
| 英文摘要 | Mammalian AlkB homologue 2 (ALKBH2) is the primary housekeeping DNA demethylase, effectively repairing endogenously ormed methylated lesions in double-stranded DNA. Our previous studies demonstrated that a hydrophobic β-hairpin otif of ALKBH2 could play crucial roles in base-pair stability interrogation and damaged base flipping. Using chemical ross-linking strategy, we obtained two crystal structures of human ALKBH2 mutant bound to duplex DNA. The structural nalysis suggests that the β-hairpin motif is flexible in conformation and is likely to slide along the DNA duplex in local regions o search for damaged base. This study provides a new mechanistic insight into DNA damage detection by ALKBH2. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/293209]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.复旦大学 2.中国科学院上海药物研究所 |
| 推荐引用方式 GB/T 7714 | Chen Baoen,Gan Jianhua,Yang Caiguang. The complex structures of ALKBH2 mutants cross-linked to dsDNA reveal the conformational swing of β-hairpin[J]. Science China. Chemistry,2014,57(2):307. |
| APA | Chen Baoen,Gan Jianhua,&Yang Caiguang.(2014).The complex structures of ALKBH2 mutants cross-linked to dsDNA reveal the conformational swing of β-hairpin.Science China. Chemistry,57(2),307. |
| MLA | Chen Baoen,et al."The complex structures of ALKBH2 mutants cross-linked to dsDNA reveal the conformational swing of β-hairpin".Science China. Chemistry 57.2(2014):307. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。