Apoptosis induced by β-amyloid25-35 in acetylcholinesteraseoverexpressing neuroblastoma cells
文献类型：期刊论文
| 作者 | Zhang Haiyan2 ; Stephen Brimijoin1; Tang Xican2
|
| 刊名 | 中国药理学报：英文版
 |
| 出版日期 | 2003 |
| 卷号 | 24.0期号:009页码:853 |
| 关键词 | Aβ25-35 乙酰胆碱酶 神经细胞瘤 细胞凋亡 MTT法 阿海默滋疾病 |
| ISSN号 | 1671-4083 |
| 英文摘要 | AIM: To examine the relationship between apoptosis induced by β-amyloid fragment 25-35 (Aβ25-35) and the activ-ity of acetylcholinesterase (ACHE) in AChE over-expresser-SC42 cells. METHODS: Cell survival was measuredby microscopy and MTT reduction; DNA laddering was observed by electrophoresis; AChE activity was determined by spectrophotometry. RESULTS: Aβ25-35 1 μmol/L exposure for 24-48 h caused a significant decrease in cell viability, along with changes in morphology and DNA fragmentation. AChE activity was affected in an inversemanner, increasing gradually to a level that was 1.7-fold higher than control at the 48-h time point. No change in thecytotoxicity of Aβ25-35 was observed when the increased AChE activities were effectively inhibited by huperzine Athroughout the 48-h exposure period. CONCLUSION: Although Aβ25-35 can induce apoptosis in SC42 cells andsimultaneously increase AChE activity, the capacity of AChE to hydrolyze acetylcholine is not involved in thisapoptosis model. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/293500]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.Department of Molecular Pharmacology, Mayo Clinic, 200 First St SW 2.中国科学院上海药物研究所 |
| 推荐引用方式 GB/T 7714 | Zhang Haiyan,Stephen Brimijoin,Tang Xican. Apoptosis induced by β-amyloid25-35 in acetylcholinesteraseoverexpressing neuroblastoma cells[J]. 中国药理学报：英文版,2003,24.0(009):853. |
| APA | Zhang Haiyan,Stephen Brimijoin,&Tang Xican.(2003).Apoptosis induced by β-amyloid25-35 in acetylcholinesteraseoverexpressing neuroblastoma cells.中国药理学报：英文版,24.0(009),853. |
| MLA | Zhang Haiyan,et al."Apoptosis induced by β-amyloid25-35 in acetylcholinesteraseoverexpressing neuroblastoma cells".中国药理学报：英文版 24.0.009(2003):853. |
入库方式： OAI收割
来源：上海药物研究所
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