Trimer procyanidin oligomers contribute to the protective effects of cinnamon extracts on pancreatic β-cells in vitro
文献类型：期刊论文
| 作者 | Sun Peng3; Wang Ting3; Chen Lu2; Yu Bangwei3; Jia Qi2; Chen Kaixian3 ; Fan Huimin1; Li Yiming2; Wang Heyao3
|
| 刊名 | Acta Pharmacologica Sinica
 |
| 出版日期 | 2016 |
| 卷号 | 37期号:8页码:1083 |
| 关键词 | cinnamon procyanidin oligomers cinnamtannin B1 procyanidin C1 cinnamtannin D1 diabetes pancreatic β-cells cultured murine islets lipotoxicity ROS insulin secretion |
| ISSN号 | 1671-4083 |
| 英文摘要 | Aim: Cinnamon extracts rich in procyanidin oligomers have shown to improve pancreatic β-cell function in diabetic db/db mice. The aim of this study was to identify the active compounds in extracts from two species of cinnamon responsible for the pancreatic β-cell protection in vitro. Methods: Cinnamon extracts were prepared from Cinnamomum tamala(CT-E) and Cinnamomum cassia(CC-E). Six compounds procyanidin B2(cpd1),(–)-epicatechin(cpd2), cinnamtannin B1(cpd3), procyanidin C1(cpd4), parameritannin A1(cpd5) and cinnamtannin D1(cpd6) were isolated from the extracts. INS-1 pancreatic β-cells were exposed to palmitic acid(PA) or H_2O_2 to induce lipotoxicity and oxidative stress. Cell viability and apoptosis as well as ROS levels were assessed. Glucose-stimulated insulin secretion was examined in PA-treated β-cells and murine islets. Results: CT-E, CC-E as well as the compounds, except cpd5, did not cause cytotoxicity in the β-cells up to the maximum dosage using in this experiment. CT-E and CC-E(12.5–50 μg/mL) dose-dependently increased cell viability in both PA- and H_2O_2-treated β-cells, and decreased ROS accumulation in H_2O_2-treated β-cells. CT-E caused more prominent β-cell protection than CC-E. Furthermore, CT-E(25 and 50 μg/mL) dose-dependently increased glucose-stimulated insulin secretion in PA-treated β-cells and murine islets, but CC-E had little effect. Among the 6 compounds, trimer procyanidins cpd3, cpd4 and cpd6(12.5–50 μmol/L) dose-dependently increased the cell viability and decreased ROS accumulation in H_2O_2-treated β-cells. The trimer procyanidins also increased glucose-stimulated insulin secretion in PA-treated β-cells. Conclusion: Trimer procyanidins in the cinnamon extracts contribute to the pancreatic β-cell protection, thus to the anti-diabetic activity. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/293591]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.同济大学 2.上海中医药大学 3.中国科学院上海药物研究所 |
| 推荐引用方式 GB/T 7714 | Sun Peng,Wang Ting,Chen Lu,et al. Trimer procyanidin oligomers contribute to the protective effects of cinnamon extracts on pancreatic β-cells in vitro[J]. Acta Pharmacologica Sinica,2016,37(8):1083. |
| APA | Sun Peng.,Wang Ting.,Chen Lu.,Yu Bangwei.,Jia Qi.,...&Wang Heyao.(2016).Trimer procyanidin oligomers contribute to the protective effects of cinnamon extracts on pancreatic β-cells in vitro.Acta Pharmacologica Sinica,37(8),1083. |
| MLA | Sun Peng,et al."Trimer procyanidin oligomers contribute to the protective effects of cinnamon extracts on pancreatic β-cells in vitro".Acta Pharmacologica Sinica 37.8(2016):1083. |
入库方式： OAI收割
来源：上海药物研究所
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