Chinese medicine CGA formula ameliorates DMN- induced liver fibrosis in rats via inhibiting MMP2/9, TIMP1/2 and the TGF-β/Smad signaling pathways
文献类型：期刊论文
| 作者 | Li Xuemei2; Peng Jinghua2; Sun Zhaolin1; Tian Huajie2; Duan Xiaohua2; Liu Lin2; Ma Xin2; Feng Qin2; Liu Ping2; Hu Yiyang2 |
| 刊名 | 中国药理学报：英文版
 |
| 出版日期 | 2016 |
| 卷号 | 000期号:006页码:783 |
| 关键词 | TGF-β1 肝纤维化 配方改进 信号通路 CGA DMN 大鼠 中药 |
| ISSN号 | 1671-4083 |
| 英文摘要 | Aim： Chinese medicine CGA formula consists of polysaccharide from Cordyceps sinensis mycelia （CS-PS）, gypenosides and amygdalin, which is derived from Fuzheng Huayu （FZHY） capsule for treating liver fibrosis. In this study we attempted to confirm the therapeutic effects of CGA formula in dimethylnitrosamine （DMN）-induced liver fibrosis in rats, and to identify the mechanisms of anti-fibrotic actions. Methods： Rats were injected with DMN （10 mg.kg-l.d-1, ip） for 3 consecutive days per week over a 4-week period. The rats then were orally administered with CGA formula （CS-PS 60 mg.kg-1.-1, gypenosides 50 mg.kg-1.d-1 and amygdalin 80 mg.kg-1.d-1） daily in the next 2 weeks. CS-PS, gypenosides or amygdalin alone were administered as individual component controls, whereas colchicine and FZHY were used as positive controls. Serum biomarkers were measured. Hepatic injury, collagen deposition and stellate cell activation were examined. The MMP activities, expression of TIMP protein and proteins involved in the TGF-β1/Smad signaling pathways in liver tissues were assayed. Results： In DMN-treated rats, administration of CGA formula significantly decreased serum ALT, AST and total bilirubin and hepatic hydroxyproline levels, increased serum albumin level, and attenuated liver fibrosis as shown by histological examination. Furthermore, these effects were comparable to those caused by administration of FZHY, and superior to those caused by administration of colchicine or the individual components of CGA formula. Moreover, administration of CGA formula significantly decreased the protein levels of a-SMA, TGF-β1, TGF-β1 receptor （TI3R-I）, p-T-R-I, p-TI3R-II, p-Smad2, p-Smad3, TIMP1 and TIMP2, as well as MMP2 and MMP9 activities in liver tissues of DMN-treated rats. Conclusion： Chinese medicine CGA formula ameliorates DMN-induced liver fibrosis in rats, and this effect was likely associated with the down-regulation of MMP2/9 activities, TIMP1/2 protein expression and the TGF-β1/Smad signaling pathways in the liver. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/293941]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.中国科学院上海药物研究所 2.上海中医药大学 |
| 推荐引用方式 GB/T 7714 | Li Xuemei,Peng Jinghua,Sun Zhaolin,et al. Chinese medicine CGA formula ameliorates DMN- induced liver fibrosis in rats via inhibiting MMP2/9, TIMP1/2 and the TGF-β/Smad signaling pathways[J]. 中国药理学报：英文版,2016,000(006):783. |
| APA | Li Xuemei.,Peng Jinghua.,Sun Zhaolin.,Tian Huajie.,Duan Xiaohua.,...&Hu Yiyang.(2016).Chinese medicine CGA formula ameliorates DMN- induced liver fibrosis in rats via inhibiting MMP2/9, TIMP1/2 and the TGF-β/Smad signaling pathways.中国药理学报：英文版,000(006),783. |
| MLA | Li Xuemei,et al."Chinese medicine CGA formula ameliorates DMN- induced liver fibrosis in rats via inhibiting MMP2/9, TIMP1/2 and the TGF-β/Smad signaling pathways".中国药理学报：英文版 000.006(2016):783. |
入库方式： OAI收割
来源：上海药物研究所
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