Requirement of PSD-95 for dopamine D_1 receptor modulating glutamate NR1a/NR2B receptor function~1
文献类型：期刊论文
| 作者 | Weihua GU; Shen YANG; Weixing SHI; Guozhang JIN ; Xuechu ZHEN
|
| 刊名 | Acta Pharmacologica Sinica
 |
| 出版日期 | 2007 |
| 卷号 | 28期号:6页码:756 |
| 关键词 | D_1 dopamine receptor NMDA PSD-95 signal transduction calcium |
| ISSN号 | 1671-4083 |
| 英文摘要 | Aim: To elucidate the role of scaffold protein postsynaptic density (PSD)-95 in the dopamine D_1 receptor (D_1R)-modulated NR1a/NR2B receptor response. Methods: The human embryonic kidney 293 cells expressing D_1R (tagged with the enhanced yellow fluorescent protein) and NR1a/NR2B with or without co-expression of PSD-95 were used in the experiments. The Ca2+ influx measured by imaging technique was employed to monitor N -methyl-D-aspartic acid receptors (NMDAR) function. Results: The application of dopamine (DA, 100 μmol/L) did not alter glutamate/glycine (Glu/Gly)-induced NMDAR-mediated Ca~(2+) influx in cells only expressing the D_1R/NR1a/NR2B receptor. However, DA increased Glu/Gly-induced Ca2+ influx in a concentration-dependent manner while the cells were co-expressed with PSD-95. D1R-stimulated Ca~(2+) influx was inhibited by a selective D1R antagonist SCH23390. Moreover, pre-incubation with either the protein kinase A (PKA) inhibitor H89, or the protein kinase C (PKC) inhibitor chelerythrine attenuated D_1R-enhanced Ca~(2+) influx induced by the N-methyl-D-aspartic acid (NMDA) agonist. The results clearly indicate that D_1R-modulated NR1a/NR2B receptor function depends on PSD-95 and is subjected to the regulation of PKA and PKC. Conclusion: The present study provides the first evidence that PSD-95 is essential in D_1R-regulated NR1a/NR2B receptor function. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/294150]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 中国科学院上海药物研究所 |
| 推荐引用方式 GB/T 7714 | Weihua GU,Shen YANG,Weixing SHI,et al. Requirement of PSD-95 for dopamine D_1 receptor modulating glutamate NR1a/NR2B receptor function~1[J]. Acta Pharmacologica Sinica,2007,28(6):756. |
| APA | Weihua GU,Shen YANG,Weixing SHI,Guozhang JIN,&Xuechu ZHEN.(2007).Requirement of PSD-95 for dopamine D_1 receptor modulating glutamate NR1a/NR2B receptor function~1.Acta Pharmacologica Sinica,28(6),756. |
| MLA | Weihua GU,et al."Requirement of PSD-95 for dopamine D_1 receptor modulating glutamate NR1a/NR2B receptor function~1".Acta Pharmacologica Sinica 28.6(2007):756. |
入库方式： OAI收割
来源：上海药物研究所
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