Protective effects of β-dihydroagarofuran-type sesquiterpene against Aβ_(25-35)-induced neuronal apoptosis and oxidative damage
文献类型：期刊论文
| 作者 | Ji Shasha2; Lei Yun1; Huang Xiaotian1; Gao Zhiqin2 |
| 刊名 | Journal of Chinese Pharmaceutical Sciences
 |
| 出版日期 | 2016 |
| 卷号 | 25期号:8页码:582 |
| 关键词 | 阿尔茨海默病 β-淀粉样蛋白 神经保护 细胞凋亡 氧化应激 |
| ISSN号 | 1003-1057 |
| 英文摘要 | Excessive beta-amyloid (Aβ) plays a detrimental role in the pathogenesis of Alzheimer's disease (AD), which is closely associated with apoptosis and oxidative stress in neurons. Therefore, identification of active small molecules with potent effects on neutralizing Aβ-induced neurotoxicity would be a promising strategy for delaying or preventing AD progression. In the present study, we discovered that pretreatment with CF-1 ((1R,2S,4R,5S,7R,9S,10S)-1,15-diacetoxy-2-benzoyloxy-9-cinnamoyloxy- β-di-hydroagarofuran), a sesquiterpene isolated from the seeds of Celastrus flagellaris, attenuated Aβ_(25-35)-induced reduction in cell viability in a concentration-dependent manner, as evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Above neuroprotective effect of CF-1 was associated with a significant decrease of apoptotic cells as measured by 4,6-diamidino-2-phenylindole (DAPI) staining, which concurrently happened with marked inhibition in the level of cleaved Caspase-3, an apoptotic executive protein. CF-1 pretreatment also markedly reduced the intracellular accumulation of reactive oxygen species (ROS) following Aβ exposure in SH-SY5Y neuroblastoma cells, but such pretreatment had no notable influence on 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging. In conclusion, we demonstrated that a novel natural product, CF-1, possessed promising effects against Aβ-induced neuronal apoptosis and oxidative stress, which could be a potential drug lead or candidate for the treatment of Aβ-associated neurotoxicity. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/294300]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.中国科学院上海药物研究所 2.潍坊医学院 |
| 推荐引用方式 GB/T 7714 | Ji Shasha,Lei Yun,Huang Xiaotian,et al. Protective effects of β-dihydroagarofuran-type sesquiterpene against Aβ_(25-35)-induced neuronal apoptosis and oxidative damage[J]. Journal of Chinese Pharmaceutical Sciences,2016,25(8):582. |
| APA | Ji Shasha,Lei Yun,Huang Xiaotian,&Gao Zhiqin.(2016).Protective effects of β-dihydroagarofuran-type sesquiterpene against Aβ_(25-35)-induced neuronal apoptosis and oxidative damage.Journal of Chinese Pharmaceutical Sciences,25(8),582. |
| MLA | Ji Shasha,et al."Protective effects of β-dihydroagarofuran-type sesquiterpene against Aβ_(25-35)-induced neuronal apoptosis and oxidative damage".Journal of Chinese Pharmaceutical Sciences 25.8(2016):582. |
入库方式： OAI收割
来源：上海药物研究所
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