WSS45, a sulfated α-D-glucan, strongly interferes with Dengue 2 virus infection in vitro
文献类型：期刊论文
| 作者 | Tong Xiankun3 ; Qiu Hong2; Zhang Xin1; Shi Liping3; Wang Guifeng3; Ji Feihong3; Ding Huiyong3; Tang Wei3; Ding Kan2; Zuo Jianping3
|
| 刊名 | Acta Pharmacologica Sinica
 |
| 出版日期 | 2010 |
| 卷号 | 31期号:5页码:585 |
| 关键词 | Dengue virus sulfated polysaccharide infection BHK cells α-D-glucan |
| ISSN号 | 1671-4083 |
| 英文摘要 | Aim: To investigate the mode of action of WSS45, one sulfated derivative of an α-D-glucan from the Gastrodia elata BI, on the multiplication cycle of dengue virus serotype 2 (DV2), including initial infection and intracellular replication. Methods: Virus multiplication in BHK cells were monitored by qRT-PCR, plaque reduction assay, and flow cytometry. Initial virus infection was dissected into adsorption and penetration steps by converting temperature and treating by acid glycine. Surface bound virions were detected by immunofluorescence staining for Evelope protein. Results: WSS45 effectively inhibited DV2 infection in BHK cells with an EC_(50) value of 0.68±0.17 μg/mL, mainly interfered with virus adsorption, in a very early stage of the Virus cycle. However, WSS45 showed no viricidal effect. Moreover, WSS45 could increase the detaching of virus from cell surface in BHK cell line. Conclusion: WSS45 exerted potent inhibitory effect on DV2 through interfering with the interaction between viruses and targeted cells. This activity was related to its molecular size. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/294385]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.Institution Center for Disease Control and Prevention of Guangdong Province 2.中国科学院 3.中国科学院上海药物研究所 |
| 推荐引用方式 GB/T 7714 | Tong Xiankun,Qiu Hong,Zhang Xin,et al. WSS45, a sulfated α-D-glucan, strongly interferes with Dengue 2 virus infection in vitro[J]. Acta Pharmacologica Sinica,2010,31(5):585. |
| APA | Tong Xiankun.,Qiu Hong.,Zhang Xin.,Shi Liping.,Wang Guifeng.,...&Zuo Jianping.(2010).WSS45, a sulfated α-D-glucan, strongly interferes with Dengue 2 virus infection in vitro.Acta Pharmacologica Sinica,31(5),585. |
| MLA | Tong Xiankun,et al."WSS45, a sulfated α-D-glucan, strongly interferes with Dengue 2 virus infection in vitro".Acta Pharmacologica Sinica 31.5(2010):585. |
入库方式： OAI收割
来源：上海药物研究所
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