Fibrinogen interaction of CHO cells expressing chimeric αⅡb/αvβ3 integrin
文献类型：期刊论文
| 作者 | Juanjuan CHEN3; Xiaoyu SU2; Xiaodong XI2; Liping LIN3; Jian DING3 ; He LU1
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| 刊名 | 中国药理学报：英文版
 |
| 出版日期 | 2008 |
| 卷号 | 29.0期号:002页码:204 |
| 关键词 | 纤维蛋白原 信号转换 细胞粘附 分子机制 |
| ISSN号 | 1671-4083 |
| 英文摘要 | Aim： The molecular mechanisms of the affinity regulation of αvβ3 integrin are important in tumor development, wound repairing, and angiogenesis. It has been established that the cytoplasmic domains of αvβ3 integrin play an important role in integrin-ligand affinity regulation. However, the relationship of structure-function within these domains remains unclear. Methods： The extracellular and transmembrane domain of αⅡb was fused to the αv integrin cytoplasmic domain, and the chimeric α subunit was coexpressed in Chinese hamster ovary （CHO） cells with the wild-type β3 subunit or with 3 mutant β3 sequences bearing truncations at the positions of T741, Y747, and F754, respectively. The CHO cells expressing these recombinant integrins were tested for soluble fibrinogen binding and the cell adhesion and spreading on immobilized fibrinogen. Results： All 4 types of integrins bound soluble fibrinogen in the absence of agonist stimulation, and only the cells expressing the chimeric α subunit with the wild-type β3 subunit, but not those with truncated β3, could adhere to and spread on immobilized fibrinogen. Conclusion： The substitution αⅡb at the cytoplasmic domain with the αv cytoplasmic sequence rendered the extracellular αⅡbβ3 a constitutively activated conformation for ligands without the need of ＂inside-out＂ signals. Our results also indicated that the COOH-terminal sequence of β3 might play a key role in integrin αⅡb/αvβ3-mediated cell adhesion and spreading on immobilized fibrinogen. The cells expressing αⅡb/αvβ3 have enormous potential for facilitating drug screen- ing for antagonists either to αvβ3 intracellular interactions or to αⅡbβ3 receptor functions. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/294682]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.Saint Louis Hospital 2.Shanghai Institute of Hematology, Ruijin Hospital 3.中国科学院上海药物研究所 |
| 推荐引用方式 GB/T 7714 | Juanjuan CHEN,Xiaoyu SU,Xiaodong XI,et al. Fibrinogen interaction of CHO cells expressing chimeric αⅡb/αvβ3 integrin[J]. 中国药理学报：英文版,2008,29.0(002):204. |
| APA | Juanjuan CHEN,Xiaoyu SU,Xiaodong XI,Liping LIN,Jian DING,&He LU.(2008).Fibrinogen interaction of CHO cells expressing chimeric αⅡb/αvβ3 integrin.中国药理学报：英文版,29.0(002),204. |
| MLA | Juanjuan CHEN,et al."Fibrinogen interaction of CHO cells expressing chimeric αⅡb/αvβ3 integrin".中国药理学报：英文版 29.0.002(2008):204. |
入库方式： OAI收割
来源：上海药物研究所
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